Plant Science


    Protocols in Current Issue
    Rhizoctonia solani Infection Assay of Young Sugar Beet and Arabidopsis plantlets
    Authors:  Fredrik Dölfors, Louise Holmquist, Georgios Tzelepis and Christina Dixelius, date: 01/20/2022, view: 1318, Q&A: 0

    Rhizoctonia solani is a soil-borne fungus, which rarely produces any spores in culture. Hence, all inoculation procedures are based on mycelia, often as a coat on cereal kernels, placed in close vicinity to the plant to be infected. In this protocol, an inoculation method is described where the fungus is first allowed to infest a perlite-maize

    High-throughput Screening for Defense Priming-inducing Compounds in Parsley Cell Cultures
    Authors:  Kathrin Schmitz, Linda Werner and Uwe Conrath, date: 10/20/2021, view: 860, Q&A: 0

    Defense priming describes the enhanced potency of cells to activate defense responses. Priming accompanies local and systemic immune responses and can be triggered by microbial infection or upon treatment with certain chemicals. Thus, chemically activating defense priming is promising for biomedicine and agriculture. However, test systems for

    Tomato Stem Injection for the Precise Assessment of Ralstonia solanacearum Fitness in Planta
    Authors:  Yaru Wang, Liu Xian, Gang Yu and Alberto P. Macho, date: 08/20/2021, view: 1424, Q&A: 0

    Ralstonia solanacearum is a soil-borne pathogen with worldwide distribution that causes bacterial wilt disease in more than 250 plant species. R. solanacearum invades plants through the roots, reaches the vascular system, and colonizes the whole plant by moving through the xylem, where it eventually replicates rapidly, causing plant death. Usual

    A Fast and Easy Method to Study Ralstonia solanacearum Virulence upon Transient Gene Expression or Gene Silencing in Nicotiana benthamiana Leaves
    Authors:  Wenjia Yu and Alberto P. Macho, date: 08/05/2021, view: 1845, Q&A: 0

    Ralstonia solanacearum is a devastating soil-borne bacterial pathogen that causes disease in multiple host plants worldwide. Typical assays to measure virulence of R. solanacearum in laboratory conditions rely on soil-drenching inoculation followed by observation and scoring of disease symptoms. Here, we describe a novel inoculation protocol to

    Analysis of Soluble Sugar Content in Minute Quantities of Rice Tissues by GC-MS
    Authors:  Li-he Xu, Li-ying Xiao, Xue-qiong Xiao, Yan-nong Xiao and Gao-feng Wang, date: 07/05/2021, view: 2333, Q&A: 0

    Soluble sugars play key roles in plant growth, development, and adaption to the environment. Characterizing sugar content profiling of plant tissues promotes our understanding of the mechanisms underlying these plant processes. Several technologies have been developed to quantitate soluble sugar content in plant tissues; however, it is difficult

    Pipecolic Acid Quantification Using Gas Chromatography-coupled Mass Spectrometry
    Authors:  Keshun Yu, Huazhen Liu and Pradeep Kachroo, date: 12/05/2020, view: 2125, Q&A: 0

    Pipecolic acid (Pip), a non-proteinacious product of lysine catabolism, is an important regulator of immunity in plants and humans alike. For instance, Pip accumulation is associated with the genetic disorder Zellweger syndrome, chronic liver diseases, and pyridoxine-dependent epilepsy in humans. In plants, Pip accumulates upon pathogen infection

    Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana
    Authors:  Yanrong You, Chunpeng An and Chuanyou Li, date: 03/05/2020, view: 3141, Q&A: 0
    [Abstract] Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model ...
    Tracking Root Interactions System (TRIS) Experiment and Quality Control
    Authors:  Hassan Massalha, Elisa Korenblum, Orr H. Shapiro and Asaph Aharoni, date: 04/20/2019, view: 5125, Q&A: 0
    [Abstract] Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high ...
    Quantification of the Humidity Effect on HR by Ion Leakage Assay
    Authors:  Musoki Mwimba and Xinnian Dong, date: 04/05/2019, view: 4034, Q&A: 0
    [Abstract] We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes. This protocol quantifies tissue cell death by measuring ion leakage due to loss of membrane integrity during the ...
    Peptide Feeding and Mechanical Wounding for Tomato Seedlings
    Authors:  Lei Wang, Yan Wang and Georg Felix, date: 03/20/2019, view: 4354, Q&A: 0
    [Abstract] Plants need to respond appropriately to wounding and herbivorous insects. Peptide signals have been implicated in local and systemic induction of appropriate plant defense responses. To study these peptide signals and their perception in host plants, it is important to have reproducible bioassays. Several assays, such as treatment of peptide ...

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