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A CRISPR Competition Assay to Identify Cancer Genetic Dependencies
Authors:
Vishruth Girish
and
Jason M. Sheltzer
,
date:
07/20/2020,
view:
5686,
Q&A:
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[Abstract] The CRISPR/Cas9 system is a powerful tool for genome editing, wherein the RNA-guided nuclease Cas9 can be directed to introduce double-stranded breaks (DSBs) at a targeted locus. In mammalian cells, these DSBs are typically repaired through error-prone processes, resulting in insertions or deletions (indels) at the targeted locus. Researchers can ...
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