Fat Turnover Assay in Drosophila
Authors: Subhash D. Katewa and
Pankaj Kapahi,
date: 11/05/2016,
view: 10638,
Q&A: 0
[Abstract] Like all animals, Drosophila shows robust fat (triglyceride) turnover, i.e., they synthesize, store and utilize triglyceride for their daily metabolic needs. The protocol describes a simple assay to measure this turnover of triglycerides in Drosophila.
Measurement of Transferrin- and Non-transferrin-bound Iron Uptake by Mouse Tissues
[Abstract] Iron in blood plasma is bound to its transport protein transferrin, which delivers iron to most tissues. In iron overload and certain pathological conditions, the carrying capacity of transferrin can become exceeded, giving rise to non-transferrin-bound iron, which is taken up preferentially by the liver, kidney, pancreas, and heart. The ...
LDH-A Enzyme Assay
Authors: Di Zhao,
Yue Xiong,
Qun-Ying Lei and
Kun-Liang Guan,
date: 10/05/2013,
view: 13736,
Q&A: 0
[Abstract] LDH (Lactate dehydrogenase) enzyme catalyzes the reversible conversion of pyruvate to lactate using NAD+ as a cofactor. Although the physiological significance of lactate accumulation in tumor cells, a dead-end product in cellular metabolism, is currently a topic of debate, it has long been known that many tumor cells express a high level of LDH-A ...