Prokaryotes

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    Protocols in Current Issue
    Purification and HPLC Analysis of Cell Wall Muropeptides from Caulobacter crescentus
    Authors:  Gabriele Stankeviciute and Eric A. Klein, date: 11/05/2019, view: 3550, Q&A: 0
    [Abstract] The peptidoglycan sacculus, or cell wall, is what defines bacterial cell shape. Cell wall composition can be best characterized at the molecular level by digesting the peptidoglycan murein polymer into its muropeptide subunits and quantifying the abundance of muropeptides using high-pressure liquid chromatography. Certain features of the cell wall ...
    Bacterial Competition Assay Based on Extracellular D-amino Acid Production
    Authors:  Laura Alvarez and Felipe Cava, date: 04/05/2018, view: 7012, Q&A: 0
    [Abstract] Bacteria live in polymicrobial communities under tough competition. To persist in a specific niche many species produce toxic extracellular effectors as a strategy to interfere with the growth of nearby microbes. One of such effectors are the non-canonical D-amino acids. Here we describe a method to test the effect of D-amino acid production in ...
    Isolate and Sub-fractionate Cell Membranes from Caulobacter crescentus
    Author:  Khatira Anwari, date: 10/20/2012, view: 13358, Q&A: 1
    [Abstract] Cell membranes from Caulobacter can be isolated and separated into inner and outer membranes according their characteristic buoyant densities on a sucrose gradient. Fractionation can be used to determine the localisation of uncharacterised proteins and to enrich protein complexes present in either of these membranes for biochemical ...



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