General —Plant developmental biology —Plant Science

Histochemical Staining of Suberin in Plant Roots

Authors: Peter Marhavý and Shahid Siddique, date: 02/05/2021, view: 3694, Q&A: 1

[Abstract]

Histological stains are useful tools for characterizing cell shape, arrangement and the material they are made from. Stains can be used individually or simultaneously to mark different cell structures or polymers within the same cells, and to visualize them in different colors. Histological stains can be combined with genetically-encoded

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Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa

Authors: Aleksei Sorokin, Narendra Singh Yadav, Daniel Gaudet and Igor Kovalchuk, date: 01/05/2021, view: 3029, Q&A: 1

[Abstract]

Cannabis seed germination is an important process for growers and researchers alike. Many biotechnological applications require a reliable sterile method for seed germination. This protocol outlines a seed germination procedure for Cannabis sativa using a hydrogen peroxide (H2O2) solution as liquid germination media. In this protocol, all three

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Transient Expression Assay in Strawberry Fruits

Authors: Mengting Pi, Qi Gao and Chunying Kang, date: 06/05/2019, view: 5625, Q&A: 0

[Abstract] Strawberry, including the woodland strawberry Fragaria vesca (2x) and the cultivated strawberry (Fragaria × ananassa, 8x), has emerged as a model system for studying fruit development and ripening. Transient expression provides a quick assay for gene functions or gene interactions. In strawberry, ...

Micro-computed Tomography to Visualize Vascular Networks in Maize Stems

Authors: Akiteru Maeno and Katsutoshi Tsuda, date: 01/05/2018, view: 8353, Q&A: 0

[Abstract] Plant vascular systems in the stem connect roots with aerial organs to move solutes containing minerals, nutrients as well as signaling molecules, and therefore, they play pivotal roles in plant growth and development. However, stem vascular systems, especially in crop species, have been poorly described since they are deeply embedded in the ...

Investigating the Shape of the Shoot Apical Meristem in Bamboo Using a Superellipse Equation

Authors: Qiang Wei and Peijian Shi, date: 12/05/2017, view: 4950, Q&A: 0

[Abstract] The shoot apical meristem is the origin of bamboo wood. Its structure and morphology are important for maintaining the normal development of bamboo wood. However, the traditional method to describe the morphology of the shoot apical meristem in bamboo or other plants only depends on qualitative approaches. Here we present a protocol for precisely ...

Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light

Authors: François Bizet, Lionel X. Dupuy, Anthony Glyn Bengough, Alexis Peaucelle, Irène Hummel and Marie-Béatrice Bogeat-Triboulot, date: 07/20/2017, view: 7603, Q&A: 0

[Abstract] Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for ...

Multiplexed GuideRNA-expression to Efficiently Mutagenize Multiple Loci in Arabidopsis by CRISPR-Cas9

Authors: Julia Schumacher, Kerstin Kaufmann and Wenhao Yan, date: 03/05/2017, view: 10512, Q&A: 0

[Abstract] Since the discovery of the CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein (Cas) as an efficient tool for genome editing in plants (Li et al., 2013; Shan et al., 2013; Nekrasov et al., 2013), a large variety of applications, such as gene knock-out, knock-in or transcriptional ...

Electro-fusion of Gametes and Subsequent Culture of Zygotes in Rice

Authors: Erika Toda, Yukinosuke Ohnishi and Takashi Okamoto, date: 12/20/2016, view: 9572, Q&A: 0

[Abstract] Electro-fusion system with isolated gametes has been utilized to dissect fertilization-induced events in angiosperms, such as egg activation, zygote development and early embryogenesis, since the female gametophytes of plants are deeply embedded within ovaries. In this protocol, procedures for isolation of rice gametes, electro-fusion of gametes, ...

Strategies for Performing Dynamic Gene Perturbation Experiments in Flowers

Authors: Diarmuid S. Ó’Maoiléidigh, Emmanuelle Graciet and Frank Wellmer, date: 04/05/2016, view: 7683, Q&A: 0

[Abstract] Dissecting the gene regulatory networks (GRNs) underlying developmental processes is a central goal in biology. The characterization of the GRNs underlying flower development has received considerable attention, however, novel approaches are required to reveal temporal and spatial aspects of these GRNs. Here, we provide an overview of the options ...

In vitro Microtubule Binding Assay and Dissociation Constant Estimation

Authors: Christopher Kesten, René Schneider and Staffan Persson, date: 03/20/2016, view: 13022, Q&A: 0

[Abstract] Microtubules (MTs) support an astonishing set of versatile cellular functions ranging from cell division, vesicle transport, and cell and tissue morphogenesis in various organisms. This versatility is in large mediated by MT-associated proteins (MAPs). The neuronal MAP Tau, for example, is stabilizing MTs in axons of the vertebrate nervous system ...

Plant Science

Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat

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