Cell Biology

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    Protocols in Current Issue
    Efficient Superovulation and Egg Collection from Mice
    Authors:  Miyuki Shindo, Kenji Miyado, Woojin Kang, Maki Fukami and Mami Miyado, date: 06/05/2022, view: 547, Q&A: 0
    [Abstract]

    Superovulation is a method used to reduce the number of mice used per experiment by increasing the egg number. Conventionally, superovulation for obtaining mouse eggs involves the use of equine chorionic gonadotropin (eCG) for stimulation and human CG for induction. Female mice of the C57BL/6 inbred strain spontaneously ovulate approximately 10

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    Immunomagnetic Isolation and Enrichment of Microvascular Endothelial Cells from Human Adipose Tissue
    [Abstract]

    Human adipose tissue-resident microvascular endothelial cells are not only garnering attention for their emergent role in the pathogenesis of obesity-related metabolic disorders, but are also of considerable interest for vascular tissue engineering due, in part, to the abundant, accessible, and uniquely dispensable nature of the tissue. Here, we

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    Isolation of tdTomato Expressing Inter-follicular Epidermal Melanocytes or Keratinocytes from Mouse Tail Skin
    Authors:  Samuel Pop, Oscar Urtatiz and Catherine D. Van Raamsdonk, date: 04/20/2022, view: 1106, Q&A: 0
    [Abstract]

    The epidermis is the outermost layer of the skin. It is made up of mostly keratinocytes along with a small number of melanocytes and Langerhans cells. Melanocytes produce a pigment called melanin, which is transferred to the keratinocytes, and protects these cells from damage from UV radiation, as well as generating hair and skin colours. In this

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    Skeletal Stem Cell Isolation from Cranial Suture Mesenchyme and Maintenance of Stemness in Culture
    Authors:  Takamitsu Maruyama, Hsiao-Man Ivy Yu and Wei Hsu, date: 03/05/2022, view: 780, Q&A: 0
    [Abstract]

    Skeletal stem cells residing in the suture mesenchyme are responsible for calvarial development, homeostatic maintenance, and injury-induced repair. These naïve cells exhibit long-term self-renewal, clonal expansion, and multipotency. They possess osteogenic abilities to regenerate bones in a cell-autonomous manner and can directly replace

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    A Rapid Protocol for Direct Isolation of Osteoclast Lineage Cells from Mouse Bone Marrow
    [Abstract]

    Osteoclast lineage cells (OLCs), including osteoclast precursors (OCPs) and mature osteoclasts (MOCs), participate in bone remodeling and mediate pathologic bone loss. Thus, it is essential to obtain OLCs for exploring their molecular features in both physiological and pathological conditions in vivo. However, the conventional protocols for

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    Investigation of Microvesicle Uptake by Mouse Lung-marginated Monocytes in vitro
    Authors:  Ying Ying Tan, Kieran P. O'Dea, Brijesh V. Patel and Masao Takata, date: 02/05/2022, view: 1587, Q&A: 0
    [Abstract]

    Extracellular microvesicles (MVs) are released into the circulation in large numbers during acute systemic inflammation, yet little is known of their intravascular cell/tissue-specific interactions under these conditions. We recently described a dramatic increase in the uptake of intravenously injected MVs by monocytes marginated within the

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    Isolation of Healthy F4/80+ Macrophages from Embryonic day E13.5 Mouse Fetal Liver Using Magnetic Nanoparticles for Single Cell Sequencing
    Authors:  Kaustav Mukherjee and James J. Bieker, date: 12/05/2021, view: 1421, Q&A: 0
    [Abstract]

    In vivo erythropoiesis occurs in the erythroblast island niche (EBI), comprising of a central macrophage that attaches to and aids the maturation of erythroid progenitors into mature reticulocytes. Macrophages in hematopoietic tissue such as embryonic fetal liver are heterogeneous and express the cell surface protein F4/80. Earlier methods of

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    A Simple Method for the Isolation and in vitro Expansion of Highly Pure Mouse and Human Satellite Cells
    [Abstract]

    Satellite cells (SCs) are muscle stem cells capable of regenerating injured muscle. The study of their functional potential depends on the availability of methods for the isolation and expansion of pure SCs, which retain myogenic properties after serial passages in vitro. Here, we describe a protocol for the isolation and in vitro expansion of

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    Isolation of Primary Leydig Cells from Murine Testis
    Authors:  Jinlian Liang, Yan Tang, Hanhao Li, Jiaxin Mei, Zhen Cao, Huan Xia, Rufei Huang, Yan Yang and Yadong Huang, date: 11/20/2021, view: 1127, Q&A: 0
    [Abstract]

    In males, Leydig cells are the primary source of testosterone, which is necessary for testis development, masculinization, and spermatogenesis. Leydig cells are a valuable cellular model for basic research; thus, it is important to develop an improved method for isolation and purification of Leydig cells from testes. The available methods for

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    Isolation of CD31+ Bone Marrow Endothelial Cells (BMECs) from Mice
    Authors:  Alhaji Osman Smith, Seyram Yao Adzraku, Wen Ju, Jianlin Qiao, Kailin Xu and Lingyu Zeng, date: 11/20/2021, view: 1385, Q&A: 0
    [Abstract]

    In the bone marrow microenvironment, endothelial cells (ECs) play a pivotal role in regulating the production of both growth and inhibiting factors. They are held together by adherence molecules that interact with hematopoietic progenitor cells. The study of ECs in the hematopoietic stem cell niche is limited due to the lack of efficient protocols

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