Cell imaging —Microbial cell biology —Microbiology

Tracking the Subcellular Localization of Surface Proteins in Staphylococcus aureus by Immunofluorescence Microscopy

Authors: Salvatore J. Scaffidi, Mac A. Shebes and Wenqi Yu, date: 05/20/2021, view: 4371, Q&A: 0

[Abstract]

Surface proteins of Staphylococcus aureus and other Gram-positive bacteria play essential roles in bacterial colonization and host-microbe interactions. Surface protein precursors containing a YSIRK/GXXS signal peptide are translocated across the septal membrane at mid-cell, anchored to the cell wall peptidoglycan at the cross-wall compartment,

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Rapid Detection of Proliferative Bacteria by Electrical Stimulation

Authors: Conor LA Edwards, Dmitry Malyshev, James P Stratford and Munehiro Asally, date: 02/05/2020, view: 4057, Q&A: 0

[Abstract] Detecting live bacteria is an important task for antimicrobial susceptibility testing (AST) in the medical sector and for quality-monitoring in biological industries. Current methods for live-bacteria detection suffer limitations in speed or sensitivity. In a recent paper, we reported that electrical response dynamics in membrane potential enable ...

Study of Microbial Extracellular Vesicles: Separation by Density Gradients, Protection Assays and Labelling for Live Tracking

Authors: Carolina Coelho, Raghav Vij, Daniel Q. Smith, Nathan R. Brady, Anne Hamacher-Brady and Arturo Casadevall, date: 01/20/2020, view: 3069, Q&A: 0

[Abstract] Extracellular vesicles (EVs) are produced by all domains of life including Bacteria, Archaea and Eukarya. EVs are critical for cellular physiology and contain varied cargo: virulence factors, cell wall remodeling enzymes, extracellular matrix components and even nucleic acids and metabolites. While various protocols for isolating EVs have been ...

Imaging Cryptococcus spp. Capsule by Differential Interference Contrast Microscopy Using Percoll^®

Authors: Hugo Costa Paes, Stefânia de Oliveira Frazão, Maria Sueli Soares Felipe, Arturo Casadevall and André Moraes Nicola, date: 11/20/2019, view: 2742, Q&A: 0

[Abstract] The most important virulence factor in the Cryptococcus genus is the polysaccharide capsule. This genus includes several species that cause life-threatening invasive disease. An increase in capsule thickness is important during fungal infection. The capsule is usually imaged using India ink, and crucial insights on the dynamics of its ...

Cryo-transmission Electron Microscopy of Outer-inner Membrane Vesicles Naturally Secreted by Gram-negative Pathogenic Bacteria

Authors: Lidia Delgado, Nicolás Baeza, Carla Pérez-Cruz, Carmen López-Iglesias and Elena Mercadé, date: 09/20/2019, view: 4098, Q&A: 0

[Abstract] A protocol was developed to visualize and analyze the structure of membrane vesicles (MVs) from Gram-negative bacteria. It is now accepted that these micrometric spherical vesicles are commonly produced by cells from all three domains of life, so the protocol could be useful in the study of vesicles produced by eukaryotes and archaea as well as ...

Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy

Authors: Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche, date: 08/20/2019, view: 4056, Q&A: 0

[Abstract] All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the cell and its surroundings. Despite its name, the cell wall is a flexible, dynamic structure. ...

Systematic Quantification of GFP-tagged Protein Foci in Schizosaccharomyces pombe Nuclei

Authors: Kim Kiat Lim and Ee Sin Chen, date: 12/20/2018, view: 4281, Q&A: 0

[Abstract] DNA damage repair proteins form foci in response to DNA damaging agents. The efficiency and integrity of the DNA repair pathway of a particular eukaryotic (mutant) strain is usually determined by the number of foci formed compared with their wild-type counterpart. Conventionally, focus number is determined visually, and this low accuracy may ...

Extracting and Integrating Protein Localization Changes from Multiple Image Screens of Yeast Cells

Authors: Alex X Lu, Louis-Francois Handfield and Alan M Moses, date: 09/20/2018, view: 3990, Q&A: 0

[Abstract] The evaluation of protein localization changes in cells under diverse chemical and genetic perturbations is now possible due to the increasing quantity of screens that systematically image thousands of proteins in an organism. Integrating information from different screens provides valuable contextual information about the protein function. For ...

High-throughput Microscopic Analysis of Salmonella Invasion of Host Cells

Authors: Jakub Voznica, Jost Enninga and Virginie Stévenin, date: 09/20/2018, view: 4822, Q&A: 0

[Abstract] Salmonella is a Gram-negative bacterium causing a gastro-enteric disease called salmonellosis. During the first phase of infection, Salmonella uses its flagella to swim near the surface of the epithelial cells and to target specific site of infection. In order to study the selection criteria that determine which host cells are ...

A Microfluidic Device for Massively Parallel, Whole-lifespan Imaging of Single Fission Yeast Cells

Authors: Stephen K Jones Jr, Eric C Spivey, James R Rybarski and Ilya J Finkelstein, date: 04/05/2018, view: 6978, Q&A: 0

[Abstract] Whole-lifespan single-cell analysis has greatly increased our understanding of fundamental cellular processes such as cellular aging. To observe individual cells across their entire lifespan, all progeny must be removed from the growth medium, typically via manual microdissection. However, manual microdissection is laborious, low-throughput, and ...

Microbiology

Low-viscosity Matrix Suspension Culture for Human Colorectal Epithelial Organoids and Tumoroids

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