Protocols in Current Issue
    6-hydroxydopamine (6-OHDA) Oxidative Stress Assay for Observing Dopaminergic Neuron Loss in Caenorhabditis elegans
    Authors:  Sarah-Lena Offenburger and Anton Gartner, date: 09/20/2018, view: 5358, Q&A: 0
    [Abstract] The nematode Caenorhabditis elegans is a powerful genetic model that can be used to investigate neuronal death. Research using C. elegans has been crucial to characterize cell death programmes that are conserved in mammals. Many neuronal signaling components, such as those mediating dopaminergic neurotransmission, are preserved ...
    Electroporation of Embryonic Chick Eyes
    Authors:  Agustín Luz-Madrigal, Erika Grajales-Esquivel and Katia Del Rio-Tsonis, date: 06/20/2015, view: 11936, Q&A: 0
    [Abstract] The chick embryo has prevailed as one of the major models to study developmental biology, cell biology and regeneration. From all the anatomical features of the chick embryo, the eye is one of the most studied. In the chick embryo, the eye develops between 26 and 33 h after incubation (Stages 8-9, Hamburger and Hamilton, 1951). It originates from ...
    Ex utero Electroporation into Mouse Embryonic Neocortex
    Author:  Branden R. Nelson, date: 12/20/2013, view: 10691, Q&A: 0
    [Abstract] This technique allows highly efficient and reproducible transfer of DNA/RNA into the embryonic neocortex of rodents across multiple ages. Ex utero electroporation compliments the more technically difficult in utero electroporation technique by maximizing the number of embryos for available for a given experiment, as well as ...
    In utero Electroporation of Mouse Embryo Brains
    Author:  Xuecai Ge, date: 07/20/2012, view: 26981, Q&A: 2
    [Abstract] This is a non-invasive technique to introduce transgenes into developing brains. In this technique, DNA is injected into the lateral ventricle of the embryonic brains, and is incorporated into the cells through electroporation. Embryos then continue their development in normal conditions in vivo. The effects of genes of interest can be ...

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