Electroporation of Embryonic Chick Eyes
[Abstract] The chick embryo has prevailed as one of the major models to study developmental biology, cell biology and regeneration. From all the anatomical features of the chick embryo, the eye is one of the most studied. In the chick embryo, the eye develops between 26 and 33 h after incubation (Stages 8-9, Hamburger and Hamilton, 1951). It originates from ...
Ex utero Electroporation into Mouse Embryonic Neocortex
Author: Branden R. Nelson,
date: 12/20/2013,
view: 10691,
Q&A: 0
[Abstract] This technique allows highly efficient and reproducible transfer of DNA/RNA into the embryonic neocortex of rodents across multiple ages. Ex utero electroporation compliments the more technically difficult in utero electroporation technique by maximizing the number of embryos for available for a given experiment, as well as ...
In utero Electroporation of Mouse Embryo Brains
Author: Xuecai Ge,
date: 07/20/2012,
view: 26981,
Q&A: 2
[Abstract] This is a non-invasive technique to introduce transgenes into developing brains. In this technique, DNA is injected into the lateral ventricle of the embryonic brains, and is incorporated into the cells through electroporation. Embryos then continue their development in normal conditions in vivo. The effects of genes of interest can be ...