In vitro Cell Wall Stress Assay for Fusarium oxysporum
Authors: Elena Pérez-Nadales and
Antonio Di Pietro,
date: 09/05/2016,
view: 8894,
Q&A: 0
[Abstract] In this protocol we describe a cell wall stress assay for the fungal pathogen F. oxysporum, based on exposure to the two anionic dyes Calcofluor White (CFW) and Congo Red (CR). Both compounds have been used to exert stress upon the fungal cell wall in vitro (Perez-Nadales and Di Pietro, 2015; Perez-Nadales and Di Pietro, 2011; ...
Quantification of Chitinase Activity in Fusarium oxysporum
Authors: Carmen Ruiz-Roldan and
M. Isabel G. Roncero,
date: 08/05/2016,
view: 11658,
Q&A: 1
[Abstract] Fungal morphogenetic development requires modification and plasticity of the cell wall, which implies synthesis and remodelling of its components, including chitin and glucan. Thus chitinase and glucanase activities are crucial for cell-wall biogenesis and cell division. Quantification of chitinase activity might be useful to identify structural ...