Fungi

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    Protocols in Current Issue
    Isolation of Mitochondria from Ustilago maydis Protoplasts
    [Abstract]

    Ustilago maydis, a basidiomycete that infects Zea mays, is one of the top ten fungal models for studying DNA repair, signal transduction pathways, and dimorphic transitions, among other processes. From a metabolic point of view, U. maydis lacks fermentative capacity, pointing to mitochondria as a key player in central metabolism. Oxidative

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    Detection of Apoptosis-like Cell Death in Ustilago maydis by Annexin V-FITC Staining
    Authors:  Dibya Mukherjee, Aroni Mitra and Anupama Ghosh, date: 08/05/2018, view: 5161, Q&A: 0
    [Abstract] Programmed cell death (PCD) guides the transition between key developmental stages in many organisms. PCD also remains an important fate for many organisms upon exposure to different stress conditions. Therefore, an insight into the progression of PCD during the execution of a biological phenomenon can yield significant details of the underlying ...
    Single and Multiplexed Gene Editing in Ustilago maydis Using CRISPR-Cas9
    [Abstract] The smut fungus Ustilago maydis is an established model organism for elucidating how biotrophic pathogens colonize plants and how gene families contribute to virulence. Here we describe a step by step protocol for the generation of CRISPR plasmids for single and multiplexed gene editing in U. maydis. Furthermore, we describe the ...
    Mating and Progeny Isolation in the Corn Smut Fungus Ustilago maydis
    Authors:  Marina Nadal, Johanna E. Takach, David L. Andrews and Scott E. Gold, date: 04/20/2016, view: 9523, Q&A: 0
    [Abstract] The corn smut pathogen, Ustilago maydis (U. maydis) (DC.) Corda, is a semi-obligate plant pathogenic fungus in the phylum Basidiomycota (Alexopoulos et al., 1996). The fungus can be easily cultured in its haploid yeast phase on common laboratory media. However, to complete its sexual cycle U. maydis strictly requires its ...



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