Tissue staining —Tissue analysis —Cell Biology

Identification and Quantitation of Neutrophil Extracellular Traps in Human Tissue Sections

Authors: Coraline Radermecker, Alexandre Hego, Philippe Delvenne and Thomas Marichal, date: 09/20/2021, view: 2243, Q&A: 0

[Abstract]

Neutrophils are one of the first innate immune cells recruited to tissues during inflammation. An important function of neutrophils relies on their ability to release extracellular structures, known as Neutrophil Extracellular Traps or NETs, into their environment. Detecting such NETs in humans has often proven challenging for both biological

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Histological and Immunohistochemical Examination of Stem Cell Proliferation and Reepithelialization in the Wounded Skin

Authors: Rupali Gund, Ravindra Zirmire, Haarshaadri J., Gaurav Kansagara and Colin Jamora, date: 01/20/2021, view: 3779, Q&A: 0

[Abstract]

The skin is the largest organ that protects our body from the external environment and it is constantly exposed to pathogenic insults and injury. Repair of damage to this organ is carried out by a complex process involving three overlapping phases of inflammation, proliferation and remodeling. Histological analysis of wounded skin is a convenient

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Staining and Quantitative Analysis of Myelinating Oligodendrocytes in the Mouse Grey Matter

Authors: Matthew Swire and Charles ffrench-Constant, date: 10/20/2020, view: 2631, Q&A: 0

[Abstract] Oligodendrocytes generate distinct patterns of myelination throughout the CNS. Variations in myelination along axons may enable neurons to fine-tune conduction velocities and alter signal synchronisation. Here we outline a staining protocol permitting the assessment of the number and length of myelin sheaths formed by oligodendrocyte in the mouse ...

Double Labeling of PDGFR-β and α-SMA in Swine Models of Acute Kidney Injury to Detect Pericyte-to-Myofibroblast Transdifferentation as Early Marker of Fibrosis

Authors: Alessandra Stasi, Rossana Franzin, Chiara Divella, Loreto Gesualdo, Giovanni Stallone and Giuseppe Castellano, date: 10/05/2020, view: 2108, Q&A: 0

[Abstract] Growing evidences suggest that peritubular capillaries pericytes are the main source of scar-forming myofibroblasts during chronic kidney disease (CKD), as well as early phases of acute kidney injury (AKI). In a swine model of sepsis and I/R (Ischemia Reperfusion) injury-induced AKI we demonstrated that renal pericytes are able to ...

Analysis of Generalized Fibrosis in Mouse Tissue Sections with Masson’s Trichrome Staining

Authors: Diantha van de Vlekkert, Eda Machado and Alessandra d’Azzo, date: 05/20/2020, view: 5051, Q&A: 0

[Abstract] Expansion of fibrous connective tissue and abnormal deposition of extracellular matrix (ECM) are at the basis of many fibrotic diseases. Fibrosis can occur in response to both physiological and pathological cues, including wound healing, tissue remodeling/repair and inflammation. Chronic fibrosis can lead to severe tissue damage, organ failure and ...

The Chick Chorioallantoic Membrane (CAM) Assay as a Three-dimensional Model to Study Autophagy in Cancer Cells

Authors: Félice A. Janser, Patricia Ney, Marta Teixeira Pinto, Rupert Langer and Mario P. Tschan, date: 07/05/2019, view: 7028, Q&A: 0

[Abstract] The chick chorioallantoic membrane (CAM) is an extra-embryonic organ and thus well accessible for seeding and harvesting 3D cell cultures. Samples from CAM assays are suitable for protein and gene expression analysis as well as for immuno-histochemical studies. Here we present the CAM assay as a possible model to study autophagy in different types ...

Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos

Authors: Rachna Narayanan and Andrew C. Oates, date: 03/20/2019, view: 11576, Q&A: 0

[Abstract] In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non-radioactive labels that can be detected by immunohistochemistry and coupled to ...

An Improved Staining Method for Low Signal LacZ Reporter Detection in Mouse Embryos

Authors: Xiaopeng Shen, Feng Xu, Shen Wu, Meng Li, Jingyi Zhang, Rui Liang and Yu Liu, date: 03/05/2019, view: 5185, Q&A: 0

[Abstract] In many fields of biology, especially in the field of developmental biology, LacZ reporter staining is an approach used to monitor gene expression patterns. In the LacZ reporter system, the LacZ gene is inserted in the endogenous location of the target gene via gene knock-in or by constructing a transgenic cassette in ...

Immunohistochemical Staining of TLR4 in Human Skeletal Muscle Samples

Authors: Daniela Verzola, Samantha Milanesi, Francesca Costigliolo and Giacomo Garibotto, date: 01/20/2019, view: 4507, Q&A: 0

[Abstract] Growing evidence suggests the involvement of TLR4, a receptor in the innate immune system, in muscle loss in uremia. Recently, we have evaluated TLR4 in human skeletal muscle from chronic kidney disease patients, by immunohistochemistry and image analysis. Unlike the commonly-used Western blot method, immunohistochemistry allows for the ...

Mouse Mammary Gland Whole Mount Preparation and Analysis

Authors: Cornelia Tolg, Mary Cowman and Eva A. Turley, date: 07/05/2018, view: 13724, Q&A: 0

[Abstract] The mammary gland undergoes extensive remodeling during pregnancy and is also subject to neoplastic processes both of which result in histological changes of the gland epithelial structure. Since the mammary tree is a complex three-dimensional structure a method is needed that provides an overview of the entire gland. Whole mounts provide this ...

Cell Biology

Evaluation of Urine Proteins by Capillary Electrophoresis

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