Protocols in Current Issue
    Measuring Auxin Transport Capacity in Seedling Roots of Medicago truncatula
    Authors:  Jason Liang Pin Ng and Ulrike Mathesius, date: 06/20/2016, view: 8347, Q&A: 0
    [Abstract] Measurement of auxin transport capacity provides quantitative data on the physiological machinery involved in auxin transport within plants. This technique is easy to perform and gives quick results. Radiolabelled auxin (indole-3-acetic-acid) is fed into the roots of Medicago truncatula via an agar block. The resulting radioactivity from ...
    Quantifying Auxin Metabolites in Young Root Tissue of Medicago truncatula by Liquid Chromatography Electrospray-ionisation Quadrupole Time-of-flight (LC-ESI-QTOF) Tandem Mass Spectrometry
    Authors:  Jason Liang Pin Ng, Thy T. Truong, Charles H. Hocart and Ulrike Mathesius, date: 06/20/2016, view: 8772, Q&A: 0
    [Abstract] Auxins represent a major group of phytohormones controlling plant development. The spatio-temporal regulation of auxin gradients is essential for the initiation, growth and correct development of plant organs. Because auxins and their metabolites occur at trace levels in plant tissue, experiments requiring identification plus their selective and ...
    Cytohistochemical Determination of Calcium Deposition in Plant Cells
    Authors:  Wan-Jun Zhang and Tao Wang, date: 01/20/2016, view: 7072, Q&A: 0
    [Abstract] Calcium plays important roles in maintaining plant cellular structure and also acts as a key secondary messenger in intercellular signaling. Thirty years ago, methods of detecting calcium in sub-cellular level had been established (Stockwell and Hanchey, 1982; Borgers et al., 1982) and reviewed extensively (Wick and Heplerm, 1982). We had ...

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