Digestion of Peptidoglycan and Analysis of Soluble Fragments
Authors: Ryan E. Schaub and
Joseph P. Dillard,
date: 08/05/2017,
view: 10728,
Q&A: 0
[Abstract] Peptidoglycan (murein) is a vital component of the cell wall of nearly all bacteria, composed of sugars linked by short peptides. This protocol describes the purification of macromolecular peptidoglycan from cultured bacteria and the analysis of enzyme-digested peptidoglycan fragments using high performance liquid chromatography (HPLC). Digested ...
Product Analysis of Starch Active Enzymes by TLC
Authors: Darrell Cockburn and
Nicole Koropatkin,
date: 10/20/2015,
view: 11745,
Q&A: 1
[Abstract] Thin layer chromatography (TLC) is a useful technique for detecting the presence of monosaccharides through to oligosaccharides, though it needs to be optimized for the specific sugars that are analyzed. Here we present a method for visualizing the reaction product(s) of starch active enzymes, which can contain α-1, 4 linked and α-1, 6 linked ...