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A Molecular Cloning and Sanger Sequencing-based Protocol for Detecting Site-specific DNA Methylation

Authors: Wei Guo, Anthony Cannon and Damon Lisch, date: 05/05/2022, view: 489, Q&A: 0

[Abstract]

DNA methylation is a conserved chemical modification, by which methyl groups are added to the cytosine of DNA molecules. Methylation can influence gene expression without changing the sequence of a particular gene. This epigenetic effect is an intriguing phenomenon that has puzzled biologists for years. By probing the temporal and spatial patterns

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Reduced Representation Bisulfite Sequencing in Maize

Authors: Fei-Man Hsu, Chung-Ju Rachel Wang and Pao-Yang Chen, date: 03/20/2018, view: 6506, Q&A: 0

[Abstract] DNA methylation is an epigenetic modification that regulates plant development (Law and Jacobsen, 2010). Whole genome bisulfite sequencing (WGBS) is a state-of-the-art method for profiling genome-wide methylation patterns with single-base resolution (Cokus et al., 2008). However, for an organism with a large genome, e.g., the 2.1 ...

ER Microsome Preparation and Subsequent IAA Quantification in Maize Coleoptile and Primary Root Tissue

Author: Verena Kriechbaumer, date: 05/05/2016, view: 9718, Q&A: 0

[Abstract] Auxin is a major growth hormone in plants and the first plant hormone to be discovered and studied (Darwin and Darwin, 1880). The auxin molecule in plants was first identified as indole-3-acetic acid (IAA) by Kögl et al. (1934). Active research over nearly a decade has shed light on many of the molecular mechanisms of its action but the ...

Ustilago maydis Virulence Assays in Maize

Authors: Amey Redkar and Gunther Doehlemann, date: 03/20/2016, view: 10836, Q&A: 0

[Abstract] The basidiomycetous smut fungus Ustilago maydis (U. maydis) infects all aerial parts of its host plant maize (Zea mays L.). Infection symptoms are seen in the form of prominent tumors on all aerial parts of maize, after the establishment of a biotrophic interaction with the host usually around 5-6 days post infection ...

EdU Based DNA Synthesis and Cell Proliferation Assay in Maize Infected by the Smut Fungus Ustilago maydis

Authors: Amey Redkar and Gunther Doehlemann, date: 03/20/2016, view: 8529, Q&A: 0

[Abstract] The basidiomycetous smut fungus Ustilago maydis (U. maydis) infects all aerial parts of its host plant maize (Zea mays L.). Infection is seen in the form of prominent tumorous symptoms after the establishment of a biotrophic interaction with the host, usually around 5-6 days after infection. The fungus colonizes the ...

Protocol for the Generation of a Transcription Factor Open Reading Frame Collection (TFome)

Authors: John Gray, Brett Burdo, Mary P. Goetting-Minesky, Bettina Wittler, Matthew Hunt, Tai Li, David Velliquette, Julie Thomas, Tina Agarwal, Kasey Key, Irene Gentzel, Michael dos Santos Brito, Maria Katherine Mejía-Guerra, Layne N. Connolly, Dalya Qaisi, Wei Li, Maria I. Casas, Andrea I. Doseff and Erich Grotewold, date: 08/05/2015, view: 13727, Q&A: 1

[Abstract] The construction of a physical collection of open reading frames (ORFeomes) for genes of any model organism is a useful tool for the exploration of gene function, gene regulation, and protein-protein interaction. Here we describe in detail a protocol that has been used to develop the first collection of transcription factor (TF) and co-regulator ...

Measurement of Net High-affinity Urea Uptake in Maize Plants

Authors: Laura Zanin, Nicola Tomasi and Roberto Pinton, date: 06/05/2015, view: 8007, Q&A: 0

[Abstract] Despite its extensive use as a nitrogen fertilizer, the role of urea as a directly accessible nitrogen source for crop plants is still poorly understood. So far, the physiological and molecular aspects of urea acquisition have been investigated only in a few plant species highlighting the importance of a urea transporter in roots, DUR3 (Kojima et ...

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