中文
Home
Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
Plant Science
Stem Cell
Systems Biology
Archive
For Authors
Submission Procedure
Preparation Guidelines
Submit Manuscript
Editorial Process
Editorial Criteria
Publishing Ethics
Competing Interests
Copyright & Permissions
About Us
A peer-reviewed protocol journal. No publication fee; no access fee.
Search
Protozoans
Latest Protocol Video
Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat
Author Map
Categories
Protocols in Current Issue
By Date
By View
Isolation of Latex Bead Phagosomes from
Dictyostelium
for
in vitro
Functional Assays
Authors:
Ashwin D’Souza
,
Paulomi Sanghavi
,
Ashim Rai
,
Divya Pathak
and
Roop Mallik
,
date:
12/05/2016,
view:
8574,
Q&A:
0
[Abstract] We describe a protocol to purify latex bead phagosomes (LBPs) from
Dictyostelium
cells. These can be later used for various
in vitro
functional assays. For instance, we use these LBPs to understand the microtubule motor-driven transport on
in vitro
polymerized microtubules. Phagosomes are allowed to mature for defined ...
Dictyostelium
Cultivation, Transfection, Microscopy and Fractionation
Authors:
Jennifer Hirst
,
Robert R Kay
and
David Traynor
,
date:
06/05/2015,
view:
12355,
Q&A:
0
[Abstract] The real time visualisation of fluorescently tagged proteins in live cells using ever more sophisticated microscopes has greatly increased our understanding of the dynamics of key proteins during fundamental physiological processes such as cell locomotion, chemotaxis, cell division and membrane trafficking. In addition the fractionation of cells ...
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.