Protocols in Current Issue
    Electrophysiological Measurements of Isolated Blood Vessels
    Authors:  Samuel A Molina, Daniela Maier-Begandt, Brant E Isakson and Michael Koval, date: 03/20/2022, view: 440, Q&A: 0

    The lumen of blood vessels is covered by endothelial cells, which regulate their permeability to ions and solutes. Endothelial permeability depends on the vascular bed and cell phenotype, and is influenced by different disease states. Most characterization of endothelial permeability has been carried out using isolated cells in culture. While

    Live Intravital Intestine with Blood Flow Visualization in Neonatal Mice Using Two-photon Laser Scanning Microscopy

    This protocol describes a novel technique to investigate the microcirculation dynamics underlying the pathology in the small intestine of neonatal mice using two-photon laser-scanning microscopy (TPLSM). Recent technological advances in multi-photon microscopy allow intravital analysis of different organs such as the liver, brain and intestine.

    Isolation of Murine Primary Aortic Smooth Muscle Cells
    Authors:  Max Ole Hubert, Juan Rodriguez-Vita, Lena Wiedmann and Andreas Fischer, date: 02/05/2021, view: 3398, Q&A: 1

    Vascular smooth muscle cells (VSMCs) have been cultured for decades to study the role of these cells in cardiovascular disorders. The most common source of VSMCs is the rat aorta. Here we show the adaptation of this method to isolate and culture mouse aortic VSMCs. The advantage of this method is that there are many more transgenic mouse lines

    Development of the Deoxycorticosterone Acetate (DOCA)-salt Hypertensive Rat Model
    Authors:  Nayara Pestana-Oliveira, David B. Nahey, Tim Johnson and John P. Collister, date: 08/05/2020, view: 3400, Q&A: 0
    [Abstract] According to the World Health Organization (WHO), nearly 1.13 billion people worldwide have hypertension, a major factor responsible for premature death globally. The inherent multifactorial nature of hypertension makes its study difficult since the chronic rise in blood pressure depends on the intricate connection between dietary, genetic ...
    Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe
    Authors:  Farzane Sivandzade, Aditya Bhalerao and Luca Cucullo, date: 01/05/2019, view: 22077, Q&A: 0
    [Abstract] In recent years, fluorescent dyes have been frequently used for monitoring mitochondrial membrane potential to evaluate mitochondrial viability and function. However, the reproducibility of the results across laboratories strongly depends upon following well validated and reliable protocols along with the appropriate controls. Herein, we provide a ...
    Generation of BMEC Lines and in vitro BMEC-HSPC Co-culture Assays
    Authors:  Peipei Guo and Shahin Rafii, date: 11/05/2018, view: 5005, Q&A: 0
    [Abstract] Endothelial cells (ECs) sustain the self-renewal and regeneration of adult hematopoietic stem and progenitor cells (HSPCs) via deployment of EC-derived paracrine factors, termed as angiocrine factors. Generation of durable ex vivo vascular niche that maintains EC identity and preserves the angiocrine profile of organ of origin offers ...
    Isolation of Rodent Brain Vessels
    Authors:  Cristina Carvalho and Paula I. Moreira, date: 09/05/2017, view: 7512, Q&A: 0
    [Abstract] The prevalence of neurodegenerative diseases is increasing worldwide. Cerebrovascular disorders and/or conditions known to affect brain vasculature, such as diabetes, are well-known risk factors for neurodegenerative diseases. Thus, the evaluation of the brain vasculature is of great importance to better understand the mechanisms underlying brain ...
    Functional ex-vivo Imaging of Arterial Cellular Recruitment and Lipid Extravasation
    [Abstract] The main purpose of this sophisticated and highly versatile method is to visualize and quantify structural vessel wall properties, cellular recruitment, and lipid/dextran extravasation under physiological conditions in living arteries. This will be of interest for a broad range of researchers within the field of inflammation, hypertension, ...
    Vascular Smooth Muscle Cell Isolation and Culture from Mouse Aorta
    Authors:  Callie S. Kwartler, Ping Zhou, Shao-Qing Kuang, Xue-Yan Duan, Limin Gong and Dianna M. Milewicz, date: 12/05/2016, view: 18905, Q&A: 0
    [Abstract] Vascular smooth muscle cells (SMC) in the ascending thoracic aorta arise from neural crest cells, whereas SMCs in the descending aorta are derived from the presomitic mesoderm. SMCs play important roles in cardiovascular development and aortic aneurysm formation. This protocol describes the detailed process for explanting ascending and descending ...
    Aorta Ring Assay
    Authors:  Jing Jin, Xinyang Hu, Ling Zhang and Jian'an Wang, date: 07/05/2016, view: 12964, Q&A: 3
    [Abstract] Angiogenesis is the nature and pathological process of blood vessel growth from pre-existing vascular buds. It plays an important role in cancer and cardiovascular disease. The aorta ring assay is an approach to study angiogenesis. In this experiment, we used the aorta of rat as the study material, cleaned the surrounding tissue of aorta and cut ...

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