Analysis of Xyloglucan Composition in Arabidopsis Leaves
[Abstract] Xyloglucan is one of the main components of the primary cell wall in most species of plants. This protocol describes a method to analyze the composition of the enzyme-accessible and enzyme-inaccessible fractions of xyloglucan in the model species Arabidopsis thaliana. It is based on digestion with an endoglucanase that attacks ...
Whole-seed Immunolabeling of Arabidopsis Mucilage Polysaccharides
Author: Cătălin Voiniciuc,
date: 06/05/2017,
view: 6390,
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[Abstract] In addition to synthesizing and secreting copious amounts of pectic polymers (Young et al., 2008), Arabidopsis thaliana seed coat epidermal cells produce small amounts of cellulose and hemicelluloses typical of secondary cell walls (Voiniciuc et al., 2015c). These components are intricately linked and are released as a ...
Quantification of the Mucilage Detachment from Arabidopsis Seeds
Author: Cătălin Voiniciuc,
date: 05/05/2016,
view: 7214,
Q&A: 0
[Abstract] The Arabidopsis thaliana seed coat produces large amounts of cell wall polysaccharides, which swell out of the epidermal cells upon hydration of the mature dry seeds. While most mucilage polymers immediately diffuse in the surrounding solution, the remaining fraction tightly adheres to the seed, forming a dense gel-like capsule (Macquet ...
Assay of Arabinofuranosidase Activity in Maize Roots
[Abstract] Root is a perfect model for studying the mechanisms of plant cell growth. Along the root length, several zones where cells are at different stages of development can be visualized (Figure 1). The dissection of the root on these zones allows the investigation of biochemical and genetic aspects of different growth steps. Maize primary root is much ...
Detection of Hydroxyproline O-galactoside by LC/MS
[Abstract] Hydroxyproline (Hyp) O-galactosylation is a plant-specific post-translational modification found in extracellular glycoproteins such as arabinogalactan proteins (AGPs). Hyp O-galactosylation is mediated by Hyp O-galactosyltransferase (HPGT) that catalyzes the transfer of a D-galactopyranosyl residue to the hydroxyl group ...
Analysis of Starch Synthase Activities in Wheat Grains using Native-PAGE
Author: Zhongyi Li,
date: 01/20/2016,
view: 9747,
Q&A: 0
[Abstract] Starch synthases are one class of key enzymes involving in the synthesis of cereal starch, which transfer glucose from ADP-glucose to the non-reducing end of pre-existing α-(1-4)-liked glucosyl chains of amylopectin. This protocol is highly reproducible for assaying activities for starch synthase I and IIIa in wheat and barley endosperm at ...