Lipid measurement —Lipid —Biochemistry

Rapid Lipid Quantification in Caenorhabditis elegans by Oil Red O and Nile Red Staining

Authors: Nicole L. Stuhr, James D. Nhan, Amy M. Hammerquist, Bennett Van Camp, David Reoyo and Sean P. Curran, date: 03/05/2022, view: 2153, Q&A: 0

[Abstract]

The ability to stain lipid stores in vivo allows for the facile assessment of metabolic status in individuals of a population following genetic and environmental manipulation or pharmacological treatment. In the animal model Caenorhabditis elegans, lipids are stored in and mobilized from intracellular lipid droplets in the intestinal and

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Oil Red O Staining for Lipid Content in Caenorhabditis elegans

Authors: Feng-Yung Wang and Tsui-Ting Ching, date: 08/20/2021, view: 2283, Q&A: 0

[Abstract]

The nematode Caenorhabditis elegans has emerged as a popular model system for studying the regulation of lipid metabolism. Therefore, it is critical to develop a method for determining fat storage in individual worms. Oil Red O (ORO) staining has been validated as an accurate assessment for major fat storage in C. elegans. Here, we describe an

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Triacylglycerol Measurement in HeLa Cells

Authors: Ximing Du and Hongyuan Yang, date: 12/20/2020, view: 2080, Q&A: 0

[Abstract]

Lipid droplets store triacylglycerols (triglycerides) and sterol esters to regulate lipid and energy homeostasis. Triacylglycerol measurement is often performed during the investigation of lipid droplet formation and growth. This protocol describes a reliable method using a fluorometric lipid quantification kit to measure triacylglycerols

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Total Triglyceride Quantification in Caenorhabditis elegans

Authors: Anjali Sandhu and Varsha Singh, date: 11/20/2020, view: 2708, Q&A: 0

[Abstract] Several studies suggest an important role of lipid metabolism in regulating longevity of Caenorhabditis elegans. Therefore, assays to quantify lipids have enormous value in understanding aging and pathologies associated with it. Approximately 70% of lipid metabolism genes in the nematode have orthologs in humans. Amenability of C. elegans ...

Quantification of Fatty Acids in Mammalian Tissues by Gas Chromatography–Hydrogen Flame Ionization Detection

Authors: Fumie Hamano, Suzumi M Tokuoka, Tomomi Hashidate-Yoshida, Hideo Shindou, Takao Shimizu and Yoshihiro Kita, date: 05/05/2020, view: 3431, Q&A: 0

[Abstract] In mammalian organisms, fatty acids (FAs) exist mostly in esterified forms, as building blocks of phospholipids, triglycerides, and cholesteryl esters, while some exist as non-esterified free FAs. The absolute quantification of FA species in total lipids or in a specific lipid class is critical in lipid-metabolism studies. To quantify FAs in ...

GC-MS-Based Analysis of Methanol: Chloroform-extracted Fatty Acids from Plant Tissues

Authors: Manish Kumar Patel, Shubhashis Das and Jitendra Kumar Thakur, date: 09/20/2018, view: 11663, Q&A: 0

[Abstract] Fatty acids (FAs) are carboxylic acids with long aliphatic chains that may be straight, branched and saturated or unsaturated. Most of the naturally occurring plant FAs contains an even number of carbon (C4-C24). FAs are used in food and pharmacological industries due to their nutritional importance. In addition, FAs are considered as a promising ...

Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL)

Authors: Anna Worthmann, Clara John and Joerg Heeren, date: 07/05/2018, view: 4547, Q&A: 0

[Abstract] The here described method can be used to estimate the uptake of orally provided cholesterol in mice. Briefly, mice are gavaged with radiolabeled cholesterol and 4 h later, organ distribution of the radiolabel is determined by liquid scintillation counting. The method has been applied successfully to determine dietary cholesterol handling of mice ...

Tracking Lipid Transfer by Fatty Acid Isotopolog Profiling from Host Plants to Arbuscular Mycorrhiza Fungi

Authors: Andreas Keymer, Claudia Huber, Wolfgang Eisenreich and Caroline Gutjahr, date: 04/05/2018, view: 6417, Q&A: 0

[Abstract] Lipid transfer from host plants to arbuscular mycorrhiza fungi was hypothesized for several years because sequenced arbuscular mycorrhiza fungal genomes lack genes encoding cytosolic fatty acid synthase (Wewer et al., 2014; Rich et al., 2017). It was finally shown by two independent experimental approaches (Jiang et al., ...

Fatty Acid Content and Composition of Triacylglycerols of Chlorella kessleri

Authors: Motohide Aoki and Norihiro Sato, date: 01/05/2018, view: 7325, Q&A: 0

[Abstract] Triacylglycerols (TAGs) are esters formed from one glycerol and three fatty acids. TAGs are induced to accumulate in algal cells under environmental stress conditions including nutrient-limitation, hyperosmosis, and low temperature, for the storage of metabolic energy and carbon, and also for the consumption of excess energy (e.g., Hirai ...

Lipidomic Analysis of Caenorhabditis elegans Embryos

Authors: Hung-Chi Yang, Cheng-Yu Hung, Yi-Yun Pan, Szecheng J Lo and Daniel Tsun-Yee Chiu, date: 09/20/2017, view: 8277, Q&A: 0

[Abstract] Metabolomic is an emerging field of system biology. Lipidomic, a branch of metabolomic, aims to characterize lipophilic metabolites in biological systems. Caenorhabditis elegans (C. elegans) is a genetically tractable and versatile animal model for novel discovery of lipid metabolism. In addition, C. elegans embryo is ...

Biochemistry

Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat

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