ROS Detection in Botryococcus braunii Colonies with CellROX Green Reagent
[Abstract] We analyzed the reactive oxygen species (ROS) accumulation in the colony-forming green microalga Botryococcus braunii in response to several stress inducers such as NaCl, NaHCO3, salicylic acid (SA), methyl jasmonate, and acetic acid. A staining assay using the fluorescent dye CellROX Green was used. CellROX Green is a ...
Ciliary and Flagellar Membrane Vesicle (Ectosome) Purification
Author: William Dentler,
date: 06/20/2014,
view: 9110,
Q&A: 0
[Abstract] Eukaryotic cilia/flagella are ideal organelles for the analysis of membrane trafficking, membrane assembly, and the functions of a variety of signal transduction molecules. Cilia are peninsular organelles and the membrane lipids, membrane proteins, and microtubular-associated components are selectively transported into cilia through the region ...
Deflagellation and Regeneration in Chlamydomonas
Author: William Dentler,
date: 06/20/2014,
view: 13737,
Q&A: 0
[Abstract] Eukaryotic cilia/flagella are one of the only cellular structures that can be removed without injuring cells, can be highly purified for biochemical analysis, and, in many cells, can be completely reassembled within 90 minutes. Following amputation, the expression of many flagellar genes is up-regulated, and many are packaged and associated with ...
Heat Shock Treatment of Chlamydomonas reinhardtii and Chlorella Cells
[Abstract] The protocol is very reliable and simple for inducing heat shock in unicellular green algae cells. The main purpose was to compare cellular response of three Chlorella species, isolated from different habitats: Chlorella vulgaris 8/1- thermophilic, Chlorella kesslery- mesophilic and C. vulgaris- extremophilic. ...
Immunoelectron Microscopy in Chlamydomonas Cells
Authors: Maria A. Sinetova and
Alexandra G. Markelova,
date: 03/05/2013,
view: 11074,
Q&A: 0
[Abstract] The method of immunoelectron microscopy is intended for localization of proteins inside the cells of Chlamydomonas reinhardtii or other microalgae and cyanobacteria. This protocol was used to study localization of carbonic anhydrase Cah3 with antibodies raised in rabbit, though it can be used to localize any other abundant protein. ...