Cytohistochemical Determination of Calcium Deposition in Plant Cells
Authors: Wan-Jun Zhang and
Tao Wang,
date: 01/20/2016,
view: 7083,
Q&A: 0
[Abstract] Calcium plays important roles in maintaining plant cellular structure and also acts as a key secondary messenger in intercellular signaling. Thirty years ago, methods of detecting calcium in sub-cellular level had been established (Stockwell and Hanchey, 1982; Borgers et al., 1982) and reviewed extensively (Wick and Heplerm, 1982). We had ...
Histochemical Staining of Silica Body in Rice Leaf Blades
[Abstract] Silicon (Si) is a biologically important element for plants in the order Poales (Yamamoto et al., 2011; Kido et al., 2015). In rice, Si is mainly deposited in the motor cells and the cell walls of the leaf epidermis. However, the molecular basis of this overall process has not been elucidated. Thus, we propose a protocol for the ...
High-throughput Quantification of Ammonium Content in Arabidopsis
[Abstract] This protocol is a simple colorimetric assay for internal ammonium quantification in aqueous extracts from plant tissues. The method is based on the phenol hypochlorite assay (Berthelot reaction):
NH4+ + hypochlorite + OH- + phenol → indophenol
The oxidation of indophenol caused by phenol ...
Histochemical Detection of Zn in Plant Tissues
Authors: Ilya Seregin,
Anna Kozhevnikova and
Henk Schat,
date: 05/20/2015,
view: 9539,
Q&A: 0
[Abstract] Accumulation of metals in plant tissues, and occasionally, different cells of the same tissue, may be highly non-uniform (Seregin and Kozhevnikova, 2008). Easy-to-use histochemical methods may greatly help to investigate the distribution and accumulation of metals within and among plant tissues, and also provide information on their subcellular ...