Plants

Categories

    Protocols in Current Issue
    Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids
    Authors:  Nicolás Cara, Carlos F. Marfil, María V. Bertoldi and Ricardo W. Masuelli, date: 07/05/2020, view: 2908, Q&A: 0
    [Abstract] Methylation-Sensitive Amplification Polymorphism (MSAP) is a versatile marker for analyzing DNA methylation patterns in non-model species. The implementation of this technique does not require a reference genome and makes it possible to determine the methylation status of hundreds of anonymous loci distributed throughout the genome. In addition, ...
    Quantification of Ralstonia solanacearum Colonization of Potato Germplasm Using Luminescence
    Authors:  Andrea Paola Zuluaga, Núria S. Coll and Marc Valls, date: 05/05/2015, view: 9453, Q&A: 0
    [Abstract] We have developed an unsophisticated, non-disruptive and accurate method for evaluation of pathogen colonization in planta. In this protocol we use a Ralstonia solanacearum (R. solanacearum) UY031 strain genetically modified to constitutively generate light from a synthetic luxCDABE operon stably inserted in its ...
    Isolation of Mitochondria from Potato Tubers
    [Abstract] One way to study the function of plant mitochondria is to extract them from plant tissues in an uncontaminated, intact and functional form. The reductionist assumption is that the components present in such a preparation and the in vitro measurable functions or activities reliably reflect the in vivo properties of the organelle ...
    Potato Transformation
    Authors:  Demosthenis Chronis, Shiyan Chen, Ping Lang, Tien Tran, David Thurston and Xiaohong Wang, date: 01/05/2014, view: 16606, Q&A: 0
    [Abstract] This is a protocol to produce stable transgenic potato plants (Solanum tuberosum cv. Désirée) by Agrobacterium-mediated genetic transformation, which is established based on a method described by (Jung et al., 2005) with some modifications. Agrobacterium tumefaciens strain LBA4404 carrying the desired construct ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.