Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids
[Abstract] Methylation-Sensitive Amplification Polymorphism (MSAP) is a versatile marker for analyzing DNA methylation patterns in non-model species. The implementation of this technique does not require a reference genome and makes it possible to determine the methylation status of hundreds of anonymous loci distributed throughout the genome. In addition, ...
Isolation of Mitochondria from Potato Tubers
Authors: Jesper F. Havelund,
Fernanda Salvato,
Mingjie Chen,
R.S.P. Rao,
Adelina Rogowska-Wrzesinska,
Ole N. Jensen,
David R. Gang,
Jay J. Thelen and
Ian Max Møller,
date: 09/05/2014,
view: 16239,
Q&A: 3
[Abstract] One way to study the function of plant mitochondria is to extract them from plant tissues in an uncontaminated, intact and functional form. The reductionist assumption is that the components present in such a preparation and the in vitro measurable functions or activities reliably reflect the in vivo properties of the organelle ...
Potato Transformation
[Abstract] This is a protocol to produce stable transgenic potato plants (Solanum tuberosum cv. Désirée) by Agrobacterium-mediated genetic transformation, which is established based on a method described by (Jung et al., 2005) with some modifications. Agrobacterium tumefaciens strain LBA4404 carrying the desired construct ...