Prokaryotes

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    Protocols in Current Issue
    A Potent Vaccine Delivery System
    Authors:  Guangzu Zhao, Armira Azuar, Istvan Toth and Mariusz Skwarczynski, date: 04/05/2021, view: 3670, Q&A: 0
    [Abstract]

    Most vaccines require co-delivery of an adjuvant in order to generate the desired immune responses. However, many currently available adjuvants are non-biodegradable, have limited efficacy, and/or poor safety profile. Thus, new adjuvants, or self-adjuvanting vaccine delivery systems, are required. Here, we proposed a self-adjuvanting delivery

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    Activity Assays for Bacteriophage Endolysin PlyPy
    Authors:  Rolf Lood and Vincent A. Fischetti, date: 09/20/2014, view: 9599, Q&A: 0
    [Abstract] Bacterial viruses (bacteriophages) escape and kill their host by degrading the bacterial peptidoglycan layer through the mechanism of enzymes called endolysins: peptidoglycan degrading enzymes. The method included here is useful for the initial characterization of any endolysin, regardless of the specific catalytic domain (as long as the activity ...
    EMSA Analysis of DNA Binding By Rgg Proteins
    Authors:  Breah LaSarre and Michael J. Federle, date: 08/05/2013, view: 12510, Q&A: 0
    [Abstract] In bacteria, interaction of various proteins with DNA is essential for the regulation of specific target gene expression. Electrophoretic mobility shift assay (EMSA) is an in vitro approach allowing for the visualization of these protein-DNA interactions. Rgg proteins comprise a family of transcriptional regulators widespread among the ...



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