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Home
Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
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Stem Cell
Systems Biology
Articles and Issues
Current Issue
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Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation
JW
Jinyun Wu
YC
Yuqi Cai
XW
Xiaoyang Wu
YY
Yue Ying
YT
Yilin Tai
Miao He*
0 Q&A
13329 Views
Mar 5, 2021
Transcardiac perfusion with saline followed by 4% paraformaldehyde (PFA) is widely used to clear blood and preserve brain for immunostaining or
in situ
hybridization. PFA breaks into formaldehyde in solution, which cross-link protein and DNA molecules to preserve tissue and cell structure. Here we provide a step by step guide for performing this procedure in mouse.
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