Biochemistry

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    Protocols in Current Issue
    Cytopathic Effect Assay and Plaque Assay to Evaluate in vitro Activity of Antiviral Compounds Against Human Coronaviruses 229E, OC43, and NL63
    Authors:  Yanmei Hu, Chunlong Ma and Jun Wang, date: 02/05/2022, view: 1467, Q&A: 0
    [Abstract]

    Coronaviruses are important human pathogens, among which the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent for the COVID-19 pandemic. To combat the SARS-CoV-2 pandemic, there is a pressing need for antivirals, especially broad-spectrum antivirals that are active against all seven human coronaviruses (HCoVs).

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    Giant Mimiviridae CsCl Purification Protocol
    Authors:  Lionel Bertaux, Audrey Lartigue and Sandra Jeudy, date: 11/20/2020, view: 1732, Q&A: 0
    [Abstract] While different giant viruses’ purification protocols are available, they are not fully described and they use sucrose gradient that does not reach an equilibrium. Here, we report a protocol for the purification of members of the Mimiviridae family virions resulting from Acanthamoeaba castellanii infections. Viruses are ...



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