Protocols in Current Issue
    Delivery of AAV for Expression of Fluorescent Biosensors in Juvenile Mouse Hippocampus

    Genetically encoded fluorescent biosensors are versatile tools for studying brain metabolism and function in live tissue. The genetic information for these biosensors can be delivered into the brain by stereotaxic injection of engineered adeno-associated viruses (AAVs), which can selectively target different cell types depending on the capsid

    In vivo Imaging of Calcium Activities from Pancreatic β-cells in Zebrafish Embryos Using Spinning-disc Confocal and Two-photon Light-sheet Microscopy
    Authors:  Jia Zhao, Shenghui Liang, Yiwen Zhao, Weijian Zong, Elisa Tran, Liangyi Chen and Yanmei Liu, date: 12/05/2021, view: 1211, Q&A: 0

    Visualizing the function of pancreatic β-cells in vivo has been a long-sought goal for β-cell researchers. Unlike imaging of β-cells in mammalian species with conventional positron emission tomography and single-photon emission computed tomography, which only provides limited spatial-temporal resolution, transparent zebrafish

    Calcium Imaging in T Lymphocytes: a Protocol for Use with Genetically Encoded or Chemical Ca2+ Indicators
    Authors:  Amit Jairaman and Michael D. Cahalan, date: 10/05/2021, view: 1344, Q&A: 0

    Elevations in cytosolic calcium (Ca2+) drive a wide array of immune cell functions, including cytokine production, gene expression, and cell motility. Live-cell imaging of cells loaded with ratiometric chemical Ca2+ indicators remains the gold standard for visualization and quantification of intracellular Ca2+ signals; ratiometric imaging can be

    Characterizing the Two-photon Absorption Properties of Fluorescent Molecules in the 680-1300 nm Spectral Range
    Authors:  Mikhail Drobizhev, Rosana S Molina and Thomas E Hughes, date: 01/20/2020, view: 2682, Q&A: 0
    [Abstract] Two-photon laser scanning microscopy (2PLSM) is a state-of-the-art technique used for non-invasive imaging deep inside the tissue, with high 3D resolution, minimal out-of-focus photodamage, and minimal autofluorescence background. For optimal application of fluorescent probes in 2PLSM, their two-photon absorption (2PA) spectra, expressed in ...

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