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Experimental Setup for a Diffusion Bioreactor to Isolate Unculturable Soil Bacteria
Authors:
Dhiraj Kumar Chaudhary
and
Jaisoo Kim
,
date:
10/05/2019,
view:
4057,
Q&A:
0
[Abstract] Unculturable bacteria are those bacteria which proliferate in their native habitat but unable to grow or thrive in the normal laboratory media and conditions. The molecular techniques have revealed the significance of these uncultured bacteria in terms of their functional diversity and potential to produce secondary metabolites. To achieve these ...
Detachment Procedure of Bacteria from Atmospheric Particles for Flow-cytometry Counting
Authors:
Carolina M. Araya
,
Alberto Cazorla
and
Isabel Reche
,
date:
06/20/2019,
view:
3778,
Q&A:
0
[Abstract] The protocol separates bacteria from atmospheric particles, obtaining with greater precision their abundance in the atmospheric deposition. This procedure is similar to the one used to separate bacteria in streambed sediments. The detachment procedure consists of a chemical treatment with sodium pyrophosphate and Tween 20 and a physical treatment ...
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