Molecular Biology

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    Protocols in Current Issue
    Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration
    Authors:  Joni Vanneste, Thomas Vercruysse, Philip Van Damme, Ludo Van Den Bosch and Dirk Daelemans, date: 06/20/2020, view: 3118, Q&A: 0
    [Abstract] Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and which pathways are involved is of great importance. Here, we make use of CRISPR-Cas9 technology to ...
    A Quantitative Heterokaryon Assay to Measure the Nucleocytoplasmic Shuttling of Proteins
    Authors:  François McNicoll and Michaela Müller-McNicoll, date: 09/05/2018, view: 7286, Q&A: 0
    [Abstract] Many proteins appear exclusively nuclear at steady-state but in fact shuttle continuously back and forth between the nucleus and the cytoplasm. For example, nuclear RNA-binding proteins (RBPs) often accompany mRNAs to the cytoplasm, where they can regulate subcellular localization, translation and/or decay of their cargos before shuttling back to ...



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