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Pollen Germination and Pollen Tube Growth of
Arabidopsis thaliana
:
In vitro
and Semi
in vivo
Methods
HD
Hugh Dickinson
JR
Josefina Rodriguez-Enriquez
RG
Robert Grant-Downton *
2 Q&A
14570 Views
Aug 20, 2018
Studies of pollen germination and post-germination development are not only essential for understanding plant reproduction but also are an excellent model system for tip-based growth. Here we describe easy, reproducible methods for germination and growth of pollen from the model plant
Arabidopsis thaliana
in artificial conditions. Our growth system can be used both for pollen placed directly on this artificial substrate as well as for the so-called ‘semi
in vivo
’ method. This is where a pistil is cut shortly after hand-pollination and the pollen tubes grow through the plant tissue and emerge from the cut end onto the surface of the artificial medium.
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