Digestion of Peptidoglycan and Analysis of Soluble Fragments
Authors: Ryan E. Schaub and
Joseph P. Dillard,
date: 08/05/2017,
view: 10710,
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[Abstract] Peptidoglycan (murein) is a vital component of the cell wall of nearly all bacteria, composed of sugars linked by short peptides. This protocol describes the purification of macromolecular peptidoglycan from cultured bacteria and the analysis of enzyme-digested peptidoglycan fragments using high performance liquid chromatography (HPLC). Digested ...