Generation and in Planta Functional Analysis of Potato Virus Y mutants
Authors: Katja Stare,
Anna Coll,
Ion Gutiérrez-Aguirre,
Magda Tušek Žnidarič,
Maja Ravnikar,
Andreja Kežar,
Luka Kavčič,
Marjetka Podobnik and
Kristina Gruden,
date: 07/20/2020,
view: 2354,
Q&A: 0
[Abstract] Potato virus Y (PVY), the type member of the genus Potyvirus (family Potyviridae), is the most widespread virus affecting potato and is included in the top five most economically detrimental plant viruses. Recently, the structure of the PVY virion has been determined by cryo-electron microscopy, which has opened the doors to ...
Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids
[Abstract] Methylation-Sensitive Amplification Polymorphism (MSAP) is a versatile marker for analyzing DNA methylation patterns in non-model species. The implementation of this technique does not require a reference genome and makes it possible to determine the methylation status of hundreds of anonymous loci distributed throughout the genome. In addition, ...
Microplate Assay to Study Carboxypeptidase A Inhibition in Andean Potatoes
Authors: Mariana Edith Tellechea,
Javier Garcia-Pardo,
Juliana Cotabarren,
Daniela Lufrano,
Laura Bakas,
Francesc Xavier Avilés,
Walter David Obregon,
Julia Lorenzo and
Sebastián Tanco,
date: 12/05/2016,
view: 8481,
Q&A: 0
[Abstract] Metallocarboxypeptidases (MCP) are zinc-dependent exopeptidases that catalyze the hydrolysis of C-terminal amide bonds in proteins and peptides. They are involved in a wide range of physiological processes and have recently emerged as relevant drug targets in biomedicine (Arolas et al., 2007). In this context, the study and discovery of ...
In vitro CLE Peptide Bioactivity Assay on Plant Roots
Authors: Shiyan Chen and
Xiaohong Wang,
date: 12/20/2015,
view: 8874,
Q&A: 0
[Abstract] Plant CLAVATA3/ESR (CLE)-related proteins play diverse roles in plant growth and development including regulating the development of root meristem. The mature functional forms of CLE peptides are typically 12-13 amino acids (aa) in length that are derived from the conserved C-termini of their precursor proteins. Genes encoding small secreted ...
Isolation of Mitochondria from Potato Tubers
Authors: Jesper F. Havelund,
Fernanda Salvato,
Mingjie Chen,
R.S.P. Rao,
Adelina Rogowska-Wrzesinska,
Ole N. Jensen,
David R. Gang,
Jay J. Thelen and
Ian Max Møller,
date: 09/05/2014,
view: 16227,
Q&A: 3
[Abstract] One way to study the function of plant mitochondria is to extract them from plant tissues in an uncontaminated, intact and functional form. The reductionist assumption is that the components present in such a preparation and the in vitro measurable functions or activities reliably reflect the in vivo properties of the organelle ...
Potato Transformation
[Abstract] This is a protocol to produce stable transgenic potato plants (Solanum tuberosum cv. Désirée) by Agrobacterium-mediated genetic transformation, which is established based on a method described by (Jung et al., 2005) with some modifications. Agrobacterium tumefaciens strain LBA4404 carrying the desired construct ...
Grafting of Potato Plants
Author: Paula Suárez-López,
date: 12/05/2012,
view: 10613,
Q&A: 1
[Abstract] Grafting is a very useful technique for testing the transmission of long-distance signals in plants and is used in agriculture and gardening for different purposes. This protocol, based on a previously published one (Jackson et al., 1998), describes a grafting method for potato plants, which has successfully been used to test the ...