Biophysics

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    Protocols in Current Issue
    Label-free Quantification of Direct Protein-protein Interactions with Backscattering Interferometry
    Authors:  Seher Abbas and Karl-Wilhelm Koch, date: 12/20/2021, view: 1292, Q&A: 0
    [Abstract]

    The functional performance of a cell depends on how macromolecules, in particular proteins, come together in a precise orientation, how they assemble into protein complexes and interact with each other. In order to study protein-protein interactions at a molecular level, a variety of methods to investigate these binding processes yield affinity

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    Measurement of the Translational Diffusion Coefficient and Hydrodynamic Radius of Proteins by Dynamic Light Scattering
    Authors:  Ernest T. Parker and Pete Lollar, date: 10/20/2021, view: 1361, Q&A: 0
    [Abstract]

    Diffusion is a fundamental process in biological systems that governs the molecular collisions driving biochemical reactions and membrane and transport. Measurement of the diffusion coefficient and application of the Stokes-Einstein equation produces the hydrodynamic radius, which is a commonly used gauge of particle size. Additionally,

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