Mammalia

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    Protocols in Current Issue
    Purification of Globular Actin from Rabbit Muscle and Pyrene Fluorescent Assays to Investigate Actin Dynamics in vitro
    Authors:  He Sun, Yuanyuan Luo and Yansong Miao, date: 12/05/2018, view: 4600, Q&A: 0
    [Abstract] Pyrene Fluorescent Assay is established to monitor the dynamic actin nucleation, elongation, capping and disassembly in vitro. This technique provides an easy handle procedure and straightforward visual data analysis. By coupling actin purification and polymerization assays in this protocol, the readers could quickly get the affordable ...
    Measuring Rat Serum Osmolality by Freezing Point Osmometry
    Authors:  Jerneja Stare and Charles W. Bourque, date: 10/05/2016, view: 12356, Q&A: 0
    [Abstract] Blood serum or plasma osmolality is the measure of electrolyte to water balance in the body’s circulation, and is tightly regulated in physiological states in order to maintain normal levels of serum solute (Bourque, 2008). Osmolality is defined as the number of osmoles of solute per kg of water (mOsm/kg) (Dufour, 1993) and can be measured using ...



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