Protocols in Current Issue
    Copper Based Site-directed Spin Labeling of Proteins for Use in Pulsed and Continuous Wave EPR Spectroscopy
    Authors:  Kevin Singewald, James A. Wilkinson and Sunil Saxena, date: 12/20/2021, view: 1214, Q&A: 0

    Site-directed spin labeling in conjunction with electron paramagnetic resonance (EPR) is an attractive approach to measure residue specific dynamics and point-to-point distance distributions in a biomolecule. Here, we focus on the labeling of proteins with a Cu(II)-nitrilotriacetic acid (NTA) complex, by exploiting two strategically placed

    Biophysical Characterization of Iron-Sulfur Proteins
    Authors:  Bhanu P. Jagilinki, Irina Paluy, Alexei M. Tyryshkin, Vikas Nanda and Dror Noy, date: 10/20/2021, view: 1077, Q&A: 0

    Iron-sulfur proteins are primordial catalysts and biological electron carriers that today drive major metabolic pathways across all forms of life. They can access a diversity of oxidation states and can mediate electron transfer over an extended range of reduction potentials spanning more than 1 V. Depending on the protein micro-environment and

    Spin Labeling of RNA Using “Click” Chemistry for Coarse-grained Structure Determination via Pulsed Electron-electron Double Resonance Spectroscopy
    Authors:  Maria F. Vicino, Tobias Hett and Olav Schiemann, date: 05/05/2021, view: 3238, Q&A: 0

    Understanding the function of oligonucleotides on a molecular level requires methods for studying their structure, conformational changes, and internal dynamics. Various biophysical methods exist to achieve this, including the whole toolbox of Electron Paramagnetic Resonance (EPR or ESR) spectroscopy. An EPR method widely used in this regard is

    Xenopus laevis Oocytes Preparation for in-Cell EPR Spectroscopy
    Authors:  Laura John and Malte Drescher, date: 04/05/2018, view: 6282, Q&A: 0
    [Abstract] One of the most exciting perspectives for studying bio-macromolecules comes from the emerging field of in-cell spectroscopy, which enables to determine the structure and dynamics of bio-macromolecules in the cell. In-cell electron paramagnetic resonance (EPR) spectroscopy in combination with micro-injection of bio-macromolecules into Xenopus ...

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