Protocols in Current Issue
    Plasma Membrane Preparation from Lilium davidii and Oryza sativa Mature and Germinated Pollen
    Authors:  Ning Yang, Bing Han, Lingtong Liu, Hao Yang and Tai Wang, date: 05/20/2017, view: 7291, Q&A: 0
    [Abstract] Pollen germination is an excellent process to study cell polarity establishment. During this process, the tip-growing pollen tube will start elongating. The plasma membrane as the selectively permeable barrier that separates the inner and outer cell environment plays crucial roles in this process. This protocol described an efficient aqueous ...
    Phenol-based Total Protein Extraction from Lily Plant Tissues
    Authors:  Yun-Chu Chen, Yu-Chun Hsiao and Co-Shine Wang, date: 02/20/2015, view: 10154, Q&A: 0
    [Abstract] The phenol-based total protein extraction method is unique in that water-soluble components such as polyphenolic compounds and nucleic acids can be easily removed. Thus, total protein is free from contaminants and allows for high quality two–dimensional gel electrophoresis. The phenol-based extraction of total protein was used in various lily ...
    Separation of Microspores from Anthers of Lilium longiflorum (Lily) and Subsequent RNA Extraction
    Authors:  Ming-Che Liu, Yun-Chu Chen and Co-Shine Wang, date: 02/20/2015, view: 9187, Q&A: 0
    [Abstract] This protocol has been designed in order to facilitate the isolation and extraction of total RNA from microspores collected from lily anther sacs. This protocol allows the extraction of high amounts of high quality RNA, as observed in agarose gels.

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