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    Protocols in Current Issue
    Documentation of Floral Secretory Glands in Pleurothallidinae (Orchidaceae) Using Scanning Electron Microscopy (SEM)
    Authors:  Adam P. Karremans, Bertie Joan van Heuven, Rob Langelaan and Barbara Gravendeel, date: 11/20/2016, view: 8647, Q&A: 0
    [Abstract] A clear, step by step description of the treatment of orchid flowers, subtribe Pleurothallidinae, with Critical Point Drying for SEM is presented. It shows that a simple, short fixation and dehydration method prior to Critical Point Drying is sufficient to obtain good results.
    Establishment of a Symbiotic in vitro System between a Green Meadow Orchid and a Rhizoctonia-like Fungus
    Authors:  Enrico Ercole, Michele Rodda, Mariangela Girlanda and Silvia Perotto, date: 05/20/2015, view: 10688, Q&A: 0
    [Abstract] Symbiotic orchid seed germination in an in vitro system allows the growth of mycorrhizal protocorms and plantlets for scientific purposes. Orchids in nature need to establish a mycorrhizal symbiosis with fungal partners to germinate and develop into adult plants. Here we present a protocol for symbiotic germination of the terrestrial ...
    Virus-induced Gene Silencing (VIGS) in Phalaenopsis Orchids
    Authors:  Ming-Hsien Hsieh, Zhao-Jun Pan, Hsin-Hung Yeh and Hong-Hwa Chen, date: 12/20/2014, view: 11567, Q&A: 0
    [Abstract] This is a protocol to produce stable silencing efficacy and efficiency for VIGS using CymMV as a silencing vector for floral functional genomics in Phalaenopsis orchids. This protocol is established based on a method created by Lu et al. (2007), and then modified by Hsieh et al. (2013a; 2013b), Lu et al. (2012) ...



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