Cell Biology

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    Protocols in Current Issue
    High-speed Atomic Force Microscopy Observation of Internal Structure Movements in Living Mycoplasma
    Authors:  Kohei Kobayashi, Noriyuki Kodera and Makoto Miyata, date: 03/05/2022, view: 1719, Q&A: 0
    [Abstract]

    Dozens of Mycoplasma species belonging to the class Mollicutes bind to solid surfaces through the organelle formed at a cell pole and glide in its direction by a unique mechanism. In Mycoplasma mobile, the fastest gliding species in Mycoplasma, the force for gliding is generated by ATP hydrolysis on an internal structure. However, the spatial and

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    Spherical Invasion Assay: A Novel Method to Measure Invasion of Cancer Cells
    [Abstract] The invasion of tumor cells into the neighboring blood vessels and lymph nodes is a vital step for distant metastasis. Traditionally, the invasive activity of growth factors (or the anti-invasive activity of drugs) is measured with the Boyden chamber assay. However, this assay has a few disadvantages like poor physiological relevance of transwell ...
    Studying Chemotactic Migration in Dunn Chamber: An Example Applied to Adherent Cancer Cells
    Authors:  Khedidja Benseddik and Kossay Zaoui, date: 02/05/2022, view: 1282, Q&A: 0
    [Abstract]

    Cell migration is a vital process in the development of multicellular organisms. When deregulated, it is involved in many diseases such as inflammation and cancer metastisation. Some cancer cells could be stimulated using chemoattractant molecules, such as growth factor Heregulin β1. They respond to the attractant or repellent gradients

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    HiSAT: A Novel Method for the Rapid Diagnosis of Allergy
    Authors:  Hirotomo Shibaguchi and Yuki Yasutaka, date: 02/05/2022, view: 1172, Q&A: 0
    [Abstract]

    To identify causative substances for allergies to drugs or foods, the lymphocyte transformation test (LTT) is currently widely used as in vitro test, but its accuracy is not satisfactory. We have developed a novel method designated high-sensitivity allergy test (HiSAT) for determining allergy expression by measuring cell kinetics, using the

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    Static Adhesion Assay for Human Peripheral Blood Mononuclear Cells
    Authors:  Giulia Vanoni, Camilla Jandus and Sara Trabanelli, date: 01/05/2022, view: 1617, Q&A: 0
    [Abstract]

    Blood endothelial cells (ECs) constitute the primary physical barrier to be crossed by circulating leukocytes, once attracted to a site of ongoing inflammation/infection. Upon a pro-inflammatory stimulus, such as tumor necrosis factor (TNF), ECs upregulate adhesion molecule expression to favor the adhesion and, subsequently, the transendothelial

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    Assessing Swarming of Aerobic Bacteria from Human Fecal Matter
    Authors:  Arjun S. Byju, Deeti Patel, Weijie Chen and Sridhar Mani, date: 05/05/2021, view: 2418, Q&A: 0
    [Abstract]

    Swarming – swift movement across a surface via flagella propulsion – is a unique property of many bacteria. The role of swarming, particularly among bacterial populations of the human gut microbiome, is not yet fully understood; although, it is becoming an area of increased scientific and clinical inquiry. To further characterize

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    An Image-based Dynamic High-throughput Analysis of Adherent Cell Migration
    [Abstract]

    In this protocol, we describe a method to monitor cell migration by live-cell imaging of adherent cells. Scratching assay is a common method to investigate cell migration or wound healing capacity. However, achieving homogenous scratching, finding the optimal time window for end-point analysis and performing an objective image analysis imply, even

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    Analysis of B Cell Migration by Intravital Microscopy
    [Abstract]

    During immune responses, B cells home to lymph nodes (LN), where they encounter antigens. Homing starts with capture and L-selectin-dependent rolling on the activated endothelium of high endothelial venules (HEV). After recognition of chemokines presented on HEV, activation of B cell integrins occurs mediating firm arrest. Subsequently, B cells

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    Single Cell Migration Assay Using Human Breast Cancer MDA-MB-231 Cell Line
    Authors:  David M. Gau and Partha Roy, date: 04/20/2020, view: 4929, Q&A: 0
    [Abstract] Cell migration is a fundamental cellular process that plays a crucial role in many physioglogical and pathological processes such as wound healing or cancer metastasis. Many assays have been developed to examine cell migration, such as the wound healing or scratch assay, Boyden Chamber or transwell assay, and the method we will describe here, ...
    Analysis of Random Migration of Cancer Cells in 3D
    Authors:  Sai P. Visweshwaran and Alexis Gautreau, date: 01/05/2020, view: 4552, Q&A: 1
    [Abstract] The ability of cancer cells to migrate through a complex three-dimensional (3D) environment is a hallmark event of cancer metastasis. Therefore, an in vitro migration assay to evaluate cancer cell migration in a 3D setting is valuable to examine cancer progression. Here, we describe such a simple migration assay in a 3D ...



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