Prokaryotes

Categories

    Protocols in Current Issue
    Extraction and Electrophoretic Analysis of Bacterial Lipopolysaccharides and Outer Membrane Proteins
    Authors:  Yue-Jia Lee and Thomas J. Inzana, date: 12/20/2021, view: 1563, Q&A: 0
    [Abstract]

    Lipopolysaccharides (LPS) (or lipooligosaccharides [LOS], which lack the O-antigen side chains characteristic of LPS), and outer membrane proteins (OMP) are major cell-surface molecules in the outer membrane (OM) of gram-negative bacteria. The LPS is responsible for causing endotoxic shock in infected hosts and, in conjunction with some OMPs,

    ...
    Generation of Non-typeable Haemophilus influenzae Directed Gene Deletion Mutants
    Author:  Jeroen D. Langereis, date: 03/05/2014, view: 10012, Q&A: 0
    [Abstract] Directed deletion mutants in non-typeable Haemophilus influenzae can be made by allelic exchange of the target gene with an artificial DNA construct in which an antibiotic resistance cassette is placed between two ~1,000 bp DNA sequences that are identical to the 5' and 3' flanking regions of the target gene. The artificial DNA construct ...
    Generation and Screening of a Non-typeable Haemophilus influenzae Tn-seq Mutant Library
    Author:  Jeroen D. Langereis, date: 03/05/2014, view: 10630, Q&A: 0
    [Abstract] The genome-wide screen Tn-seq (van Opijnen et al., 2009) is very valuable tools to identify bacterial genes with a conditionally essential function, for instance genes involved in bacterial virulence. These techniques are based on the generation of a random mutant library, which is grown in a control of challenge situation (Figure 1). The ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.