Protocols in Current Issue
    Extraction and Electrophoretic Analysis of Bacterial Lipopolysaccharides and Outer Membrane Proteins
    Authors:  Yue-Jia Lee and Thomas J. Inzana, date: 12/20/2021, view: 1563, Q&A: 0

    Lipopolysaccharides (LPS) (or lipooligosaccharides [LOS], which lack the O-antigen side chains characteristic of LPS), and outer membrane proteins (OMP) are major cell-surface molecules in the outer membrane (OM) of gram-negative bacteria. The LPS is responsible for causing endotoxic shock in infected hosts and, in conjunction with some OMPs,

    Generation of Non-typeable Haemophilus influenzae Directed Gene Deletion Mutants
    Author:  Jeroen D. Langereis, date: 03/05/2014, view: 10012, Q&A: 0
    [Abstract] Directed deletion mutants in non-typeable Haemophilus influenzae can be made by allelic exchange of the target gene with an artificial DNA construct in which an antibiotic resistance cassette is placed between two ~1,000 bp DNA sequences that are identical to the 5' and 3' flanking regions of the target gene. The artificial DNA construct ...
    Generation and Screening of a Non-typeable Haemophilus influenzae Tn-seq Mutant Library
    Author:  Jeroen D. Langereis, date: 03/05/2014, view: 10630, Q&A: 0
    [Abstract] The genome-wide screen Tn-seq (van Opijnen et al., 2009) is very valuable tools to identify bacterial genes with a conditionally essential function, for instance genes involved in bacterial virulence. These techniques are based on the generation of a random mutant library, which is grown in a control of challenge situation (Figure 1). The ...

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