Plant Science

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    Protocols in Current Issue
    Fractionation and Extraction of Crude Nuclear Proteins From Arabidopsis Seedlings
    Authors:  Jiajia Zhao, Jinsong Bao and Feifei Xu, date: 01/20/2022, view: 1534, Q&A: 0
    [Abstract]

    The plant nucleus is an important subcellular organelle that contains the genome, ribosomal RNA, and regulatory proteins, and performs a central role in the functioning and metabolism of the cell. Fractionation of intact nuclei is a crucial process to elucidate the function of nuclear proteins. Here, we present a simple method for the

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    Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy
    Authors:  Rosa L. López-Marqués and Thomas G. Pomorski, date: 11/20/2021, view: 1943, Q&A: 0
    [Abstract]

    Eukaryotic cells use a diverse set of transporters to control the movement of lipids across their plasma membrane, which drastically affects membrane properties. Various tools and techniques to analyze the activity of these transporters have been developed. Among them, assays based on fluorescent phospholipid probes are particularly suitable,

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    Preparation and Transfection of Populus tomentosa Mesophyll Protoplasts
    Authors:  Hou-Ling Wang, Ting Wang, Qi Yang, Weilun Yin, Xinli Xia, Hongwei Guo and Zhonghai Li, date: 11/20/2021, view: 1931, Q&A: 0
    [Abstract]

    Mesophyll protoplasts freshly isolated from leaves are a useful research system in plants. However, cell walls in woody plants contain more pectin, making mesophyll protoplasts isolation difficult in Populus. This has limited their application in biochemical, molecular, cellular, genetic, genomic, transcriptomic, and proteomic assays. In this

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    Enrichment of Cytoplasmic RNA Granules from Arabidopsis Seedlings
    Authors:  Zhen Lei, Eun Yu Kim and Jungnam Cho, date: 11/05/2021, view: 1485, Q&A: 0
    [Abstract]

    RNA granules (RGs) are membraneless intracellular compartments that play important roles in the post-transcriptional control of gene expression. Stress granules (SGs) are a type of RGs that form under environmental challenges and/or internal cellular stresses. Stress treatments lead to strong mRNAs translational inhibition and storage in SGs until

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    High-throughput Screening for Defense Priming-inducing Compounds in Parsley Cell Cultures
    Authors:  Kathrin Schmitz, Linda Werner and Uwe Conrath, date: 10/20/2021, view: 1067, Q&A: 0
    [Abstract]

    Defense priming describes the enhanced potency of cells to activate defense responses. Priming accompanies local and systemic immune responses and can be triggered by microbial infection or upon treatment with certain chemicals. Thus, chemically activating defense priming is promising for biomedicine and agriculture. However, test systems for

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    A Novel Method to Map Small RNAs with High Resolution
    Authors:  Kun Huang, Feray Demirci, Blake C. Meyers and Jeffrey L. Caplan, date: 08/20/2021, view: 2073, Q&A: 0
    [Abstract]

    Analyzing cellular structures and the relative location of molecules is essential for addressing biological questions. Super-resolution microscopy techniques that bypass the light diffraction limit have become increasingly popular to study cellular molecule dynamics in situ. However, the application of super-resolution imaging techniques to detect

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    Peptide-mediated Targeting of Nanoparticles with Chemical Cargoes to Chloroplasts in Arabidopsis Plants
    Authors:  Israel Santana, Peiguang Hu, Su-Ji Jeon, Chris Castillo, Hann Tu and Juan Pablo Giraldo, date: 06/20/2021, view: 2279, Q&A: 0
    [Abstract]

    Plant nanobiotechnology is a flourishing field that uses nanomaterials to study and engineer plant function. Applications of nanotechnology in plants have great potential as tools for improving crop yield, tolerance to disease and environmental stress, agrochemical delivery of pesticides and fertilizers, and genetic modification and transformation

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    A Pulse–chase EdU Method for Detection of Cell Division Orientation in Arabidopsis and Juncus prismatocarpus Leaf Primordia
    Authors:  Xiaofeng Yin and Hirokazu Tsukaya, date: 01/05/2021, view: 2267, Q&A: 0
    [Abstract] In plants, the morphological diversity of leaves is largely determined by cell division, especially cell division orientation. Whereas cell division itself is easily monitored, the detection and quantification of cell division orientation are difficult. The few existing methods for detection and quantification of cell division orientation are ...
    Lipid Droplet Isolation from Arabidopsis thaliana Leaves
    Authors:  Yovanny Izquierdo, Rubén Fernández-Santos, Tomás Cascón and Carmen Castresana, date: 12/20/2020, view: 2360, Q&A: 0
    [Abstract]

    Lipid droplets (LDs) are neutral lipid aggregates surrounded by a phospholipid monolayer and specific proteins. In plants, they play a key role as energy source after seed germination, but are also formed in vegetative tissues in response to developmental or environmental conditions, where their functions are poorly understood. To elucidate these,

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    Acidified Blue Ink-staining Procedure for the Observation of Fungal Structures Inside Roots of Two Disparate Plant Lineages
    Authors:  Jill Kowal, Elena Arrigoni and Sophie Lane, date: 10/20/2020, view: 2411, Q&A: 0
    [Abstract] Identifying microscopic mycorrhizal fungal structures in roots, i.e., hyphae, vesicles and arbuscules, requires root staining procedures that are often time consuming and involves chemicals known to present health risks from exposure. By modifying established protocols, our root staining method stains roots using a safe ink- and ...



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