Viruses

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    Protocols in Current Issue
    Selection of Vaccinia Virus Recombinants Using CRISPR/Cas9
    Authors:  Anjali Gowripalan, Stewart A. Smith and David C. Tscharke, date: 12/20/2021, view: 1053, Q&A: 0
    [Abstract]

    The engineering of poxvirus genomes is fundamental to primary and applied virology research. Indeed, recombinant poxviruses form the basis for many novel vaccines and virotherapies but producing and purifying these viruses can be arduous. In recent years, CRISPR/Cas9 has become the favoured approach for genome manipulation due to its speed and

    ...
    Human Blood Component Vaccinia Virus Neutralization Assay
    Authors:  Laura Evgin and John Bell, date: 12/05/2015, view: 7733, Q&A: 0
    [Abstract] Many therapeutic viruses, such as oncolytic viruses, vaccines, or gene therapy vectors, may be administered by the intravenous route to maximize their delivery to target tissues. Blood components, such as antibody, complement and blood cells (such as neutrophils, monocytes, T cells, B cells or platelets) may result in viral neutralization and ...
    Construction of Deletion-knockout Mutant Fowlpox Virus (FWPV)
    Authors:  Stephen M. Laidlaw and Michael A. Skinner, date: 05/20/2014, view: 7643, Q&A: 0
    [Abstract] The construction of deletion-knockout poxviruses is a useful approach to determining the function of specific virus genes. This protocol is an adaptation of the transient dominant knockout selection protocol published by Falkner and Moss (1990) for use with vaccinia virus. The protocol makes use of the dominant selectable marker Escherichia coli ...



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