Fish

Categories

    Protocols in Current Issue
    Colocalization Analysis for Cryosectioned and Immunostained Tissue Samples with or without Label Retention Expansion Microscopy (LR-ExM) by JACoP
    Authors:  Xiang Zhao, Jason Garcia, Loic A. Royer and Su Guo, date: 03/05/2022, view: 1841, Q&A: 0
    [Abstract]

    Asymmetric cell division (ACD) is fundamental for balancing cell proliferation and differentiation in metazoans. During active neurogenesis in the developing zebrafish forebrain, radial glia progenitors (RGPs) mainly undergo ACD to produce one daughter with high activity of Delta/Notch signaling (proliferative cell fate) and another daughter with

    ...
    Tracking Moving Cells in 3D Time Lapse Images Using 3DeeCellTracker
    Authors:  Chentao Wen and Koutarou D. Kimura, date: 02/20/2022, view: 1269, Q&A: 0
    [Abstract]

    Recent advancements in 3D microscopy have enabled scientists to monitor signals of multiple cells in various animals/organs. However, segmenting and tracking the moving cells in three-dimensional time-lapse images (3D + T images), to extract their dynamic positions and activities, remains a considerable bottleneck in the field. We developed a deep

    ...
    A Behavioural Assay to Investigate Judgment Bias in Zebrafish
    Authors:  Felipe Espigares, Raquel R. Martins and Rui F. Oliveira, date: 02/20/2022, view: 822, Q&A: 1
    [Abstract]

    In this protocol, we describe for the first time a judgment bias paradigm to phenotype the way zebrafish assess ambiguous stimuli. We have developed and validated a protocol for a judgment bias test based on a Go/No-go task, and performed using a half radial maze. After a habituation phase, fish are trained to discriminate between two reference

    ...
    Large-scale Analysis of Sleep in Zebrafish
    Authors:  Daniel A. Lee, Grigorios Oikonomou and David A. Prober, date: 02/05/2022, view: 1476, Q&A: 0
    [Abstract]

    Over the past decade, zebrafish have emerged as a powerful model for the study of vertebrate sleep and wake behaviors. Experimental evidence has demonstrated behavioral, anatomical, genetic, and pharmacological conservation of sleep between zebrafish and mammals, suggesting that discoveries in zebrafish can inform our understanding of mammalian

    ...
    Trichloroacetic Acid Fixation and Antibody Staining of Zebrafish Larvae
    Authors:  E. Anne Martin, Sundas Ijaz, Alberto E. Pereda and Adam C. Miller, date: 01/20/2022, view: 955, Q&A: 0
    [Abstract]

    Larval zebrafish have been established as an excellent model for examining vertebrate biology, with many researchers using the system for neuroscience. Controlling a fast escape response of the fish, the Mauthner cells and their associated network are an attractive model, given their experimental accessibility and fast development, driving

    ...
    Total Nucleic Acid Extraction from Single Zebrafish Embryos for Genotyping and RNA-seq
    Authors:  Neha Wali, Munise Merteroglu, Richard J. White and Elisabeth M. Busch-Nentwich, date: 01/05/2022, view: 1538, Q&A: 0
    [Abstract]

    RNA sequencing allows for the quantification of the transcriptome of embryos to investigate transcriptional responses to various perturbations (e.g., mutations, infections, drug treatments). Previous protocols either lack the option to genotype individual samples, or are laborious and therefore difficult to do at a large scale. We have developed a

    ...
    Wounding Zebrafish Larval Epidermis by Laceration
    Authors:  Andrew S. Kennard, Christopher K. Prinz, Ellen C. Labuz and Julie A. Theriot, date: 12/20/2021, view: 1317, Q&A: 0
    [Abstract]

    Wound healing is a critical process for maintaining the integrity of tissues, driven in large part by the active migration of cells to cover damaged regions. While the long-term tissue injury response over hours and days has been extensively studied, the rapid early migratory response of cells to injury in vivo is still being uncovered, especially

    ...
    Label-free Quantification of Direct Protein-protein Interactions with Backscattering Interferometry
    Authors:  Seher Abbas and Karl-Wilhelm Koch, date: 12/20/2021, view: 1292, Q&A: 0
    [Abstract]

    The functional performance of a cell depends on how macromolecules, in particular proteins, come together in a precise orientation, how they assemble into protein complexes and interact with each other. In order to study protein-protein interactions at a molecular level, a variety of methods to investigate these binding processes yield affinity

    ...
    In vivo Imaging of Calcium Activities from Pancreatic β-cells in Zebrafish Embryos Using Spinning-disc Confocal and Two-photon Light-sheet Microscopy
    Authors:  Jia Zhao, Shenghui Liang, Yiwen Zhao, Weijian Zong, Elisa Tran, Liangyi Chen and Yanmei Liu, date: 12/05/2021, view: 1259, Q&A: 0
    [Abstract]

    Visualizing the function of pancreatic β-cells in vivo has been a long-sought goal for β-cell researchers. Unlike imaging of β-cells in mammalian species with conventional positron emission tomography and single-photon emission computed tomography, which only provides limited spatial-temporal resolution, transparent zebrafish

    ...
    Measurement of DNA Damage Using the Neutral Comet Assay in Cultured Cells
    Authors:  Elena Clementi, Zuzana Garajova and Enni Markkanen, date: 11/20/2021, view: 1760, Q&A: 0
    [Abstract]

    Maintenance of DNA integrity is of pivotal importance for cells to circumvent detrimental processes that can ultimately lead to the development of various diseases. In the face of a plethora of endogenous and exogenous DNA damaging agents, cells have evolved a variety of DNA repair mechanisms that are responsible for safeguarding genetic

    ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.