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    Protocols in Current Issue
    Visualization and Purification of Caenorhabditis elegans Germ Granule Proteins Using Proximity Labeling
    Authors:  Hannah L. Hertz, Ian F. Price and Wen Tang, date: 04/20/2022, view: 1034, Q&A: 0
    [Abstract]

    Membraneless organelles, such as germ granules and stress granules, are liquid-like condensates formed by phase transition. Recently, we and others have adopted proximity-based labeling methods to determine the composition of these membraneless compartments. Here, we describe the use of TurboID—an engineered promiscuous biotin

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    Multiplexing Thermotaxis Behavior Measurement in Caenorhabditis elegans
    Authors:  Stephan Raiders, Mason Klein and Aakanksha Singhvi, date: 04/05/2022, view: 684, Q&A: 0
    [Abstract]

    Thermotaxis behaviors in C. elegans exhibit experience-dependent plasticity of thermal preference memory. This behavior can be assayed either at population level, on linear temperature gradients, or at the individual animal level, by radial isothermal or microfluidic tracking of orientation. These behaviors are low-throughput as well as

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    TUNEL Labeling to Detect Double-stranded DNA Breaks in Caenorhabditis elegans Gonads
    Authors:  Peter A. Kropp, Kyle Rhodehouse and Andy Golden, date: 03/20/2022, view: 413, Q&A: 0
    [Abstract]

    Analysis of DNA double strand breaks (DSBs) is important for understanding dyshomeostasis within the nucleus, impaired DNA repair mechanisms, and cell death. In the C. elegans germline, DSBs are important indicators of all three above-mentioned conditions. Although multiple methods exist to assess apoptosis in the germline of C. elegans, direct

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    Rapid Lipid Quantification in Caenorhabditis elegans by Oil Red O and Nile Red Staining
    [Abstract]

    The ability to stain lipid stores in vivo allows for the facile assessment of metabolic status in individuals of a population following genetic and environmental manipulation or pharmacological treatment. In the animal model Caenorhabditis elegans, lipids are stored in and mobilized from intracellular lipid droplets in the intestinal and

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    Tracking Moving Cells in 3D Time Lapse Images Using 3DeeCellTracker
    Authors:  Chentao Wen and Koutarou D. Kimura, date: 02/20/2022, view: 1254, Q&A: 0
    [Abstract]

    Recent advancements in 3D microscopy have enabled scientists to monitor signals of multiple cells in various animals/organs. However, segmenting and tracking the moving cells in three-dimensional time-lapse images (3D + T images), to extract their dynamic positions and activities, remains a considerable bottleneck in the field. We developed a deep

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    Flow Cytometry Analysis of Planarian Stem Cells Using DNA and Mitochondrial Dyes
    Authors:  Mohamed Mohamed Haroon, Praveen Kumar Vemula and Dasaradhi Palakodeti, date: 01/20/2022, view: 931, Q&A: 0
    [Abstract]

    Planarians are free-living flatworms that emerged as a crucial model system to understand regeneration and stem cell biology. The ability to purify neoblasts, the adult stem cell population of planaria, through fluorescence-activated cell sorting (FACS) has tremendously increased our understanding of pluripotency, specialization, and

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    Quantification of Bacterial Loads in Caenorhabditis elegans
    [Abstract]

    Caenorhabditis elegans is a ubiquitous free-living nematode that feeds on bacteria. The organism was introduced into a laboratory setting in the 1970s and has since gained popularity as a model to study host-bacteria interactions. One advantage of using C. elegans is that its intestine can be colonized by the bacteria on which it feeds.

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    Live-cell Imaging and Analysis of Germline Stem Cell Mitosis in Caenorhabditis elegans
    Authors:  Réda M. Zellag, Yifan Zhao and Abigail R. Gerhold, date: 01/05/2022, view: 1239, Q&A: 0
    [Abstract]

    Model organisms offer the opportunity to decipher the dynamic and complex behavior of stem cells in their native environment; however, imaging stem cells in situ remains technically challenging. C. elegans germline stem cells (GSCs) are distinctly accessible for in situ live imaging but relatively few studies have taken advantage of this

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    Preference Test of Plutella xylostella Larvae upon DMNT Treatment
    Authors:  Chen Chen, Zhen Tao, Chuanhong Wang, Tengyue Wang, Hongyi Chen, Taoshan Jiang and Peijin Li, date: 11/05/2021, view: 885, Q&A: 0
    [Abstract]

    We describe a method to test the preference of insects in response to (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT). We use a device that includes a horizontal glass tube, two grooves (with activated carbon), air flow, rubber stoppers/tubes, transparent glass containers (optional), and a holder for the glass tube (optional). Equal amounts of activated

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    Simultaneous Monitoring Cytoplasmic Calcium Ion and Cell Surface Phosphatidylserine in the Necrotic Touch Neurons of Caenorhabditis elegans
    Authors:  Yoshitaka Furuta and Zheng Zhou, date: 10/20/2021, view: 837, Q&A: 0
    [Abstract]

    Calcium ions trigger many cellular events, including the release of neurotransmitters at the synaptic terminal and excitotoxic cell death. Recently, we have discovered that a transient increase in the level of cytoplasmic Ca2+ triggers the exposure of phosphatidylserine (PS) on the surfaces of necrotic cells in the nematode Caenorhabditis elegans.

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