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    Cell Cycle Analysis and GFP Expression of Zebrafish Embryos by FACS
    Author:  Lili Jing, date: 02/20/2012, view: 11368, Q&A: 0
    Cell cycle analysis in combination of GFP expression has been well used to study the cell cycle distribution of GFP labeled cells. This protocol is a method to analyze the cell cycles of GFP marked cells from zebrafish embryos.
    Zebrafish Embryo DNA Preparation
    Author:  Lili Jing, date: 02/20/2012, view: 13066, Q&A: 0
    This protocol explains how to extract DNA from a single zebrafish embryo. It does not require the use of expensive kits.
    Flourescent Immunostaining Protocol for a-Bungorotoxin (AChRs) in Zebrafish
    Author:  Lili Jing, date: 02/20/2012, view: 8756, Q&A: 0
    Zebrafish embryo is convenient for studying neuromuscular system due to its transparency. This protocol is a rapid method to visualize the AChR clusters on the postsynaptic muscle membrane.
    Genotyping for Single Zebrafish (Fin Clip) or Zebrafish Embryo
    Author:  Lili Jing, date: 02/05/2012, view: 16938, Q&A: 0
    Zebrafish is increasingly used a genetic model organism in biomedical studies. This protocol provides a detailed procedure about the identification of the genotype of an adult zebrafish or a zebrafish embryo.
    Genotyping Transgenic Zebrafish Using Genomic DNA Extracted from Clutch of Embryos
    Author:  Lili Jing, date: 02/05/2012, view: 9772, Q&A: 0
    Transgenic zebrafish are very useful genetic tools to study various biological processes. Identification the right transgene founder and the subsequent transgenic animals are always tedious and time consuming. This protocol provides a relatively ...
    Preparation of Torula Yeast RNA for Hybe Solutions
    Author:  Lili Jing, date: 02/05/2012, view: 13443, Q&A: 5
    In situ hybridization in zebrafish embryos frequently suffers from high background signal. Torular yeast RNA is often added to reduce the non-specific binding that leads to the high background signal. Without appropriate preparation, ...
    In situ Hybridization in Zebrafish Embryos
    Author:  Lili Jing, date: 02/05/2012, view: 8766, Q&A: 0
    In situ hybridization is routinely used to examine the gene expression level and location of embryos. This protocol is modified from the Thisse protocol and is a detailed description of the in situ hybridization procedures in ...
    Double in situ Hybridization
    Author:  Lili Jing, date: 02/05/2012, view: 9975, Q&A: 0
    Double in situ hybridization I very useful to examine the relationship between the expression of two genes. But it is tricky because of the cross reaction of two different antibodies. This protocol is a valid method to do double color in ...
    pH3 Antibody Staining Protocol for Zebrafish
    Author:  Lili Jing, date: 01/20/2012, view: 17110, Q&A: 0
    Phosphorylation of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Staining for phosphor histone H3 is a good indicator for the proliferation of the interested cells. This protocol provides a method to ...
    Crosslinking and Immunoprecipitation in Zebrafish
    Author:  Lili Jing, date: 01/20/2012, view: 9218, Q&A: 0
    Immunoprecipitation (IP) is a routine method to detect protein binding and interactions. But the weak binding between two proteins is often hard to detect during regular IP procedure. This protocol offers a crossliking and IP combination method to ...