Mammalia

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    Quantifying Gene Expression Directly from FACS Using Hydrolysis (TaqMan) Probes on Flash-frozen Cells
    Authors:  Kirsten A. Copren, Oyang Teng, Cassandra J. Adams, Cláudia Bispo and Michael Lee, date: 04/20/2018, view: 3065, Q&A: 0
    The TaqMan Gene Expression Cells-to-CtTM Kit enables reverse transcription directly from cell lysates without the need for isolating RNA. The recommended input range for the lysis reaction is between 10-105 cells, though the ...
    Site Density Protocol
    Author:  Jun Huang, date: 07/05/2012, view: 6275, Q&A: 0
    The site densities of cell surface molecules are useful information for cell function analysis. Using antibody staining and commercial available calibration beads, this assay quantitatively determines the T cell receptor site density at the single T ...
    In vitro Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
    Author:  Jia Li, date: 11/20/2011, view: 45700, Q&A: 19
    In vitro differentiation of helper T cells of various lineages is frequently used in T helper cell study. Naïve CD4 T cells can differentiate into certain lineage of T help cells in vitro in the presence of specific stimulatory ...
    Naïve T Lymphocyte Infection by Murine Stem Cell Virus (MSCV) Protocol
    Author:  Jia Li, date: 11/20/2011, view: 10255, Q&A: 0
    This assay can be used for studying genes related to lymphocyte proliferation and differentiation in vitro. Stimulating lymphocytes to proliferate is important for the infection efficiency of MSCV. After overnight culture, if naïve cells ...
    Mouse Embryonic Stem Cell Maintenance for Differentiation
    Author:  Hogune Im, date: 10/20/2011, view: 13881, Q&A: 0
    Embryonic stem cells are derived from inner cell mass of an embryo that can differentiate into every cell type in the body. Clinically, cultured red blood cell supply is of great interest. However, some of the hurdles need to be overcome. This ...
    Culturing of C57BL/6 Mouse Embryonic Stem (ES) Cell Line
    Author:  Yuqiong Pan, date: 10/05/2011, view: 13099, Q&A: 0
    Using ATCC ES C57BL/6 as an example, it is shown here how to culture mouse embryonic stem (ES) cell line. The clonal embryonic stem cell line #693 ES C57BL/6 was derived from a strain C57BL/6J (B6) mouse blastocyst [PubMed: 11730008]. The ES cells ...
    In vivo BrdU Incorporation and Detection in Mouse
    Author:  Zheng Liu, date: 06/05/2011, view: 30772, Q&A: 0
    BrdU (Bromodeoxyuridine or 5-bromo-2’-deoxyuridine) is a synthetic nucleoside that is incorporated into DNA by proliferating cells. This protocol is to be used to incorporate and detect BrdU in murine plasma cells. The plasma cells described in this ...
    Adipocyte Subcellular Fractionation
    Author:  Nabila Aboulaich, date: 02/20/2011, view: 9164, Q&A: 0