Mammalia

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    Caesarean Sectioning and Cross-Fostering of the Mouse
    Authors:  Zheping Cai, Guohong Wang, Yang Chen and Miao He, date: 11/20/2018, view: 940, Q&A: 0
    A caesarean section is required if the recipient of an embryo transfer or any pregnant mouse has not given birth by the delivery time that is normal for the particular strain. It is commonly used to derive a strain of pathogen-free mice from a ...
    Murine xenograft model
    Author:  FengZhi Liu, date: 06/05/2012, view: 15306, Q&A: 1
    This experiment is used to test the efficacies of chemo treatments or gene therapy in an in vivo system. In this protocol, the mouse xenograft model is used.
    Isolation of Endothelial Cells from Mice
    Author:  Huan Pang, date: 04/20/2012, view: 18044, Q&A: 3
    Endothelial cells line the entire circulatory system, from the heart to the smallest capillary. In this protocol, attaining and maintaining vitality of the organ is critical. Minimizing the processing time between removing the organ and beginning ...
    Subcutaneous Injection of Tumor Cells
    Author:  Jason Reuter, date: 12/20/2011, view: 42152, Q&A: 2
    Growth of cells in the subcutaneous space of immunocompromised mice is a common method for assaying tumorigenic potential in vivo. This technique is also used to assess the effects of therapeutic interventions on cancer cell lines.
    Activation of ER-regulated Fusion Proteins In Vivo
    Author:  Jason Reuter, date: 12/20/2011, view: 9443, Q&A: 0
    Regulatable protein fusions can be made by attaching the hormone binding domain of the estrogen receptor to the N or C-terminus of a protein of interest. Activation of such ER-fusion proteins in vivo can be achieved by daily administration ...
    Glucose Tolerance Test in Mice
    Author:  Peichuan Zhang, date: 10/05/2011, view: 48369, Q&A: 5
    Glucose tolerance test is a standard procedure that addresses how quickly exogenous glucose can be cleared from blood. Specifically, uptake of glucose from the blood by cells is regulated by insulin. Impairment of glucose tolerance (i.e, ...
    Thioglycollate Induced Peritonitis
    Author:  Zheng Liu, date: 06/20/2011, view: 26032, Q&A: 3
    Intraperitoneal (i.p.) injection of thioglycollate elicits a robust influx of neutrophils into peritoneal cavity. The trafficking of the cells is believed to be mediated by chemokines CXCL1, CXCL2, and CXCL8 (Call et al., 2001; Cacalano et ...