Plant Science

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    Immunolabeling of Maize Meiocytes
    Authors:  Mateusz Zelkowski and Wojciech P. Pawlowski, date: 04/05/2020, view: 2159, Q&A: 0
    This protocol describes a quick method for immunolocalization of meiotic proteins in maize. It includes a fixation step that allows for long-term storage of material and provides good preservation of chromatin structure.
    Tandem Purification of His6-3x FLAG Tagged Proteins for Mass Spectrometry from Arabidopsis
    Authors:  He Huang and Dmitri Anton Nusinow, date: 12/05/2016, view: 10225, Q&A: 0
    Tandem affinity purification is a powerful method to identify protein complexes that function in association with a known gene of interest. This protocol describes a methodology to capture proteins tagged with His6-3x FLAG explicitly for ...
    Indirect Immunofluorescence Assay in Chlamydomonas reinhardtii
    Authors:  Takashi Yamano and Hideya Fukuzawa, date: 07/05/2016, view: 10079, Q&A: 0
    Determining the protein localization is essential to elucidate its in vivo function. Fluorescence-tagged proteins are widely used for it, but it is sometimes difficult to express tagged proteins in Chlamydomonas. Alternatively, ...
    A Phosphopeptide Purification Protocol for the Moss Physcomitrella paten
    Authors:  Xiaoqin Wang and Yikun He, date: 07/20/2015, view: 7853, Q&A: 0
    Protein phosphorylation is one of the most common post-translational modifications in eukaryotic cells and plays a critical role in a vast array of cellular processes. Efficient methods of protein extraction and phosphopeptide purification are ...
    In organello Protein Synthesis
    Authors:  Malgorzata Kwasniak-Owczarek and Hanna Janska, date: 06/20/2014, view: 11284, Q&A: 1
    In organello protein synthesis method allows the analysis of mitochondrial translation products. The principle of this method relies on incubation of isolated intact mitochondria with radiolabeled amino acids such as 35S ...
    35S pulse Labelling of Chlamydomonas Chloroplast Proteins
    Authors:  Alexandra-Viola Bohne, Christian Schwarz and Joerg Nickelsen, date: 06/05/2013, view: 8766, Q&A: 0
    35S pulse labelling of proteins is used to attach a radioactive label to newly synthesized proteins, as sulfur is an element that is mainly present in proteins (Fleischmann and Rochaix 1999). Depending on your organism’s uptake mechanisms ...



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