Biochemistry

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    qPCR of Yeast ChIP DNA
    Author:  Wei Zheng, date: 09/20/2011, view: 13110, Q&A: 1
    This protocol is adapted from Chris Yellman's personal notes for qPCR of yeast ChIP DNA to verify binding sites already identified by microarray/sequencing analysis. In addition, qPCR is a quick way to assay the quality of a ChIP sample ...
    Yeast Transcription Factor Chromatin Immunoprecipitation
    Author:  Wei Zheng, date: 07/05/2011, view: 10409, Q&A: 0
    This ChIP protocol was developed and improved over the years by various researchers in the Snyder lab, Stanford University, especially Anthony Borneman and Christopher Yellman. I have used this method to successfully map the genome-wide binding of ...
    ChIP Assay for Cell Culture
    Author:  Ran Chen, date: 01/20/2011, view: 13947, Q&A: 0
    Chromatin immunoprecipitation (ChIP) is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. Following crosslinking, cells are lysed and the DNA is broken into pieces 0.2-1.0 kb in length ...