Molecular Biology


    Cell Culture Mycoplasma Detection by PCR
    Author:  Huan Pang, date: 04/20/2012, view: 18478, Q&A: 0
    DNA was extracted from the supernatant of each sample. After PCR-amplification of mycoplasma DNA, detection was performed by gel electrophresis. The PCR primers were designed to cover the consensus sequences that can detect all types of mycoplasma ...
    Genotyping for Single Zebrafish (Fin Clip) or Zebrafish Embryo
    Author:  Lili Jing, date: 02/05/2012, view: 26497, Q&A: 0
    Zebrafish is increasingly used a genetic model organism in biomedical studies. This protocol provides a detailed procedure about the identification of the genotype of an adult zebrafish or a zebrafish embryo.
    Genotyping Transgenic Zebrafish Using Genomic DNA Extracted from Clutch of Embryos
    Author:  Lili Jing, date: 02/05/2012, view: 13596, Q&A: 0
    Transgenic zebrafish are very useful genetic tools to study various biological processes. Identification the right transgene founder and the subsequent transgenic animals are always tedious and time consuming. This protocol provides a relatively ...
    Single Worm PCR
    Author:  Fanglian He, date: 04/20/2011, view: 19655, Q&A: 1
    This protocol is used for genotyping single worms.
    Standard PCR Protocol
    Author:  Fanglian He, date: 04/05/2011, view: 37953, Q&A: 8
    This protocol describes basic steps of a PCR experiment using home-made Taq DNA polymerase. Some steps may vary with different DNA polymerase.
    Site-directed Mutagenesis Using Dpn1
    Author:  Lili Jing, date: 02/05/2011, view: 21426, Q&A: 1
    Site-directed mutagenesis is an important and widely used tool in molecular biology to generate specific changes in the DNA sequence of a given gene/genome. The protocol described here is for making nucleotide changes at specific loci in a large ...
    Colony PCR Using Yeast Spheroplasted Cells
    Author:  Bio-protocol Editor, date: 01/05/2011, view: 13232, Q&A: 1
    The first part of this protocol involves the removal of yeast cell walls using the Zymolase enzyme. The resulting spheroplast cells can then be used as template for PCR. This quick and easy to implement protocol describes how to prepare ...

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