Immunology

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    Immunophenotyping and Intracellular Staining of Fixed Whole Blood for Mass Cytometry (CyTOF)
    Authors:  Sangeeta Kowli and Holden Maecker, date: 09/05/2020, view: 3072, Q&A: 0
    In this report, we present the implementation of mass cytometry for intracellular staining using fixed whole blood. In our assay described here, 250 µl of whole blood, is stimulated in vitro with PMA/ionomycin (or left unstimulated), in the ...
    Phenotyping of Live Human PBMC using CyTOFTM Mass Cytometry
    Authors:  Michael D. Leipold and Holden Maecker, date: 01/20/2015, view: 15700, Q&A: 3
    Single-cell analysis has become an method of importance in immunology. Fluorescence flow cytometry has been a major player. However, due to issues such as autofluorescence and emission spillover between different fluorophores, alternative techniques ...
    Intracellular Cytokine Staining on PBMCs Using CyTOFTM Mass Cytometry
    Authors:  Dongxia Lin, Sheena Gupta and Holden Maecker, date: 01/05/2015, view: 19205, Q&A: 2
    In this protocol, we use a CyTOFTM mass cytometry to collect single-cell data on a large number of cytokines/chemokines as well as cell-surface proteins that characterize T cells and other immune cells. The current selected mass window in ...



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