Molecular Biology

Latest Protocol Video

Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice

Categories

    A Novel Method to Construct Binary CRISPR Vectors for Plant Transformation by Single Round of PCR Amplification
    Authors:  Kang Li, Yuhui Wang and Chuanying Fang, date: 04/05/2021, view: 2451, Q&A: 0

    CRISPR/Cas9 is an established and flexible tool for genome editing. However, most methods used to generate expression clones for the CRISPR/Cas9 are time-consuming. Hence, we have developed a one-step protocol to introduce sgRNA expression

    ...
    Histochemical Staining of Suberin in Plant Roots
    Authors:  Peter Marhavý and Shahid Siddique, date: 02/05/2021, view: 1920, Q&A: 0

    Histological stains are useful tools for characterizing cell shape, arrangement and the material they are made from. Stains can be used individually or simultaneously to mark different cell structures or polymers within the same cells, and to

    ...
    Generation and in Planta Functional Analysis of Potato Virus Y mutants
    Authors:  Katja Stare, Anna Coll, Ion Gutiérrez-Aguirre, Magda Tušek Žnidarič, Maja Ravnikar, Andreja Kežar, Luka Kavčič, Marjetka Podobnik and Kristina Gruden, date: 07/20/2020, view: 1517, Q&A: 0
    Potato virus Y (PVY), the type member of the genus Potyvirus (family Potyviridae), is the most widespread virus affecting potato and is included in the top five most economically detrimental plant viruses. Recently, the structure ...
    Assembly of Genetic Circuits with the Mammalian ToolKit
    Authors:  João P. Fonseca, Alain R Bonny, Jason Town and Hana El-Samad, date: 03/05/2020, view: 2646, Q&A: 0
    The ability to rapidly assemble and prototype cellular circuits is vital for biological research and its applications in biotechnology and medicine. The Mammalian ToolKit (MTK) is a Golden Gate-based cloning toolkit for fast, reproducible and ...
    High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach
    Authors:  Franziska M. Heydenreich, Tamara Miljuš, Dalibor Milić and Dmitry B. Veprintsev, date: 01/05/2020, view: 3073, Q&A: 0
    Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed ...
    A Refined Protocol for Identifying Citrulline-specific Monoclonal Antibodies from Single Human B Cells from Rheumatoid Arthritis Patient Material
    Authors:  Khaled Amara, Lena Israelsson, Ragnhild Stålesen, Peter Sahlström, Johanna Steen, Vivianne Malmström and Caroline Grönwall, date: 09/05/2019, view: 2988, Q&A: 0
    We describe here a detailed, refined protocol for the generation of citrulline-specific monoclonal antibodies from single human B cells from rheumatoid arthritis (RA) patients. This protocol provides a detailed guide of the procedure starting from ...
    Modifications in the Transformation Step of Commercially Available Site-directed Mutagenesis Kit Increase Its Price-performance
    Authors:  Atta Ahmad, David F. Jahad, Hamzah Karimi, Mary A. Farwell, Jean-Luc Scemama and Cindy Putnam-Evans, date: 08/05/2019, view: 2284, Q&A: 0
    Site-directed mutagenesis helps unravel the relationship between the structure and function of a protein at the level of amino acid contributions. Many techniques have been established to carry out site-directed mutagenesis on a daily routine. ...
    Efficient Assembly of Large Multiplex CRISPR/Cas9 Guide Arrays for Maize Genome Editing
    Authors:  Jarrett Man and Madelaine E. Bartlett, date: 05/05/2019, view: 4042, Q&A: 0
    CRISPR/Cas9-based genome editing in maize is an effective tool, and researchers commonly wish to target multiple genes simultaneously. Transformation of maize is currently expensive and tedious, so researchers are incentivized to build vectors that ...
    Multiple Modification of Chromosomal Loci Using URA5.3 Selection Marker in the Unicellular Red Alga Cyanidioschyzon merolae
    Authors:  Tokiaki Takemura, Sousuke Imamura, Yuki Kobayashi and Kan Tanaka, date: 04/05/2019, view: 3534, Q&A: 0
    The unicellular red alga Cyanidioschyzon merolae has been used as a eukaryotic photosynthetic model for various basic and applied studies. Although the nuclear genome of C. merolae can be modified by homologous recombination with ...
    Measuring Secretion of Capsidiol in Leaf Tissues of Nicotiana benthamiana
    Authors:  Teruhiko Kuroyanagi, Maurizio Camagna and Daigo Takemoto, date: 08/05/2018, view: 3508, Q&A: 0
    Plant species produce a wide variety of antimicrobial metabolites to protect themselves against potential pathogens in natural environments. Phytoalexins are low molecular weight compounds produced by plants in response to attempted attacks of ...



    Other Resources
    www.bio-protocol.org
    for advanced protocols
    www.bio-thing.com
    for reagents & equipment
    lab.bio-thing.cn
    for lab management
    For Advertisers
    Advertising Policy
    Advertising Integrity Board
    Advertising Terms and
    Conditions
    For Authors
    Submission Procedure
    Author guidelines
    Submit a Protocol
    About
    About Us

    Meet Our Ambassadors
    ©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
    Find out more
    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.