Biochemistry

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    In vitro Chaperone Activity Assay Using α-Amylase as Target Protein
    Authors:  Jeeshma Nambidi Parambath, Gayathri Valsala, Karthik Menon and Shiburaj Sugathan, date: 06/20/2018, view: 4457, Q&A: 0
    Small heat shock proteins (sHSP) are stress proteins which are ubiquitously found in almost all living organisms. They function as molecular chaperones, which assist in protein folding during translation and in the prevention of irreversible protein ...
    PI (phosphoinositide)-3 Kinase Assay
    Author:  Huan Pang, date: 04/20/2012, view: 9603, Q&A: 1
    PI3-kinases regulate a wide range of cellular responses through the production of phosphatidylinositol 3, 4, 5-trisphosphate [PI (3, 4, 5) P (3)] in cellular membranes. This protocol provides a highly efficient and easy-to-handle method for ...
    Extraction of Root Apoplastic Wall Fluid for Apoplastic Peroxidase Activity Assay
    Author:  Xiyan Li, date: 09/05/2011, view: 14533, Q&A: 0
    Plant roots secret a lot of peroxidases to counteract environmental influences. This protocol describes a way to extract root apoplastic wall fluid from Arabidopsis plants and to determine the peroxidase activity using guaiacol as substrate.



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